流式细胞术分析大蒜油对癌细胞DNA合成及细胞周期的影响

作者	单位
谢锦玉	中国中医研究院基础理论研究所北京 100700
高玉民	中国中医研究院基础理论研究所北京 100700
沈联慈	中国中医研究院基础理论研究所北京 100700
刘铭福	中国中医研究院基础理论研究所北京 100700
王玉萍	中国中医研究院基础理论研究所北京 100700

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中文摘要:

我们在证明大蒜油具有明显提高小鼠生命率和抑瘤率等一系列实验研究的同时,应用流式细胞术（FCM）测量腹水S₁₈₀癌细胞的脱氧核糖核酸（DNA）相对含量,以观察各时相细胞增殖周期的移行变化,发现大蒜油使DNA合成期（S）细胞明显减少,百分率下降,而DNA合成前期（G₁）细胞显著增加,说明大蒜油可阻滞癌细胞G₁期向S期的进程,造成了G₁期细胞堆积,阻断DNA合成和复制.起到抑制癌细胞的增殖周期的作用。

英文摘要:

Flow cytometry (FCM) is a new technique developed in the recent decade. This technique may measure DNA content of 5000 cells per second and trace the dynamic changes in cell proliferation cycle and offer a hint for designing clinical treatment protocol, monitor prognosis and elucidate the mechanisms of antitumor drugs. The authors previous studies showed significant effect of garlic oil on prolongation of life expectance and inhibition of tumor growth in mice. Using FCM the authors analysed the effect of garlic oil on cell cycle in S180 tumor cells, 2～6hrs after single administration or multiple administration the cell number in S phase rapidly decreased, in Gl phase increased. This suggests garlic oil may blockade cells to progress from Gl phase to S phase and result in accumulation of cells in Gl phase and directly inhibit the synthesis of DNA and the cell cycle. Theoretical basis for clinical application was offered and some aspects of antitumor mechanism of garlic oil were elucidated.

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