玉米蜂花粉对大鼠红细胞膜结构和功能的影响

作者	单位
郭贵文	山西医学院卫生毒理学教研室
柳黄	山西医学院营养与食品卫生教研室
杨琦	山西医学院营养与食品卫生教研室
高应	山西医学院生物化学教研室

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中文摘要:

本实验观察了喂饲含10%玉米蜂花粉的饲料对正常成年大鼠红细胞膜ATP酶活性及红细胞膜巯基（-SH）和唾液酸含量等的影响。实验结果表明,喂养10周后,花粉组大鼠红胞膜Na⁺,K⁺-ATP酶和Ca²⁺,Mg²⁺-ATP酶活性分为311.5±35.5和813.8±43.4nmolPi/mg protein·h,均明显高于对照组（209.9±23.9和624.9±23.3nmolPi/mgProtein·h）;红细胞膜-SH和唾液酸的含量都显著高于对照组;同时红细胞膜MDA含量明显低于对照组。结果提示,玉米蜂花粉对细胞膜的结构和功能具有较好的保护作用,这种保护作用可能是通过抑制脂质过氧化作用而实现的。

英文摘要:

The aged-related changes of ATPase activity as well as the contents of MDA. sulfhydryl and sialic acid of erythrocyte membrane had been observed in many studies. In this paper, the effect ofZea pollinium on the structure and function of erythrocyte membrane in rats was observed. 12 malerats were divided randomly into pollen and control groups, the former was fed with diet containing10% Zea pollinium; while no pollen for the latter. Results: After feeding for 10 weeks, the Na⁺, K⁺-ATPase and Ca²⁺. Mg²⁺-ATPase activities were 311.5 ± 35.5 and 813.8 ± 43.4 nmolPi/mg protein·hrespectively in pollen group. significantly higher than that of the control group （209.9 ± 23.9 and624.9 ± 23.3 nmolPi/mg protein·h）. The contents of sulfhydryl and sialic acid were also increased,but the coatent of MDA was markedly decreased with the use of Zea pollinium. These resultsindicated that Zea pollinium could inhibit formation of lipid peroxidates, protect the structure andfunction of erythrocyte membrane f the injury of peroxidate.

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