川芎嗪对急性出血坏死性胰腺炎大鼠肺损伤的作用及机制研究

作者	单位
王兴鹏	上海第二医科大学附属瑞金医院内科上海 200025
徐家裕	上海第二医科大学附属瑞金医院内科上海 200025
袁耀宗	上海第二医科大学附属瑞金医院内科上海 200025
王冠庭	上海第二医科大学附属瑞金医院内科上海 200025

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中文摘要:

急性出血坏死性胰腺炎大鼠随着时间延长,肺部郁血加重,肺系数增加;肺间质中性粒细胞浸润增多,肺间质及肺泡水肿、肺不张;电镜改变为Ⅱ型上皮细胞板层小体减少,线粒体、内质网肿胀,随着肺损伤程度加深,血浆TXB₂/6-keto-PGF_1α比值及LPO值增加。川芎嗪显著降低了TXB₂/6-keto-PGF_1α比值,降低LPO值;降低肺系数,肺组织病理形态改善,大鼠存活率提高。

英文摘要:

To evaluate the therapeutic effects and mechanisms of tetramethylpyraziue （TMP）, a Chineseherbal medicine, on the lung injury in bile-induced acute haemorrhagic necrotizing pancreatitis（AHNP） in the SD rats, the rats were randomly divided into three groups: sham-operative, untreatedand TMP treated. AHNP model were induced by ligation with 5% taurocholate. The changes of lungindex, serum lipid peroxide （LPO）, TXB₂, 6-keto-PGF₁α, and lung pathology at light and electronmicroscope were all investigated at 1, 6, 12 hours after induction of AHNP model. Survival rate ofAHNP in rats were recorded also. Results of the study showed that in untreated group, the timerelat-ed progressive pancreatic haemorrhage and necrosis, accompanied by pancreatitis--associated lunginjury, such as pronounced pulmonary congestion, alveolar and interstitial edema polymorphonu-clear granulocytes infiltration, transparent membrane formation, the density of layer body in typeⅡ endothelial cells decreasing, with some vacuole formation, mitochondria, endoplasmic reticulumswollen, basal membrane of endothelial cells rupture were observed. The level of LPO elevated at1 hour after induction of AHNP and peaked at 12 hours. TXB₂ and 6-keto-PGF₁α was increased.Using TMP treatment, survival rate increased, and lung at light and electron microscope were much improved and lung index, value of LPO, TXB₂, decreased significantly, 6-keto-PGF₁α increasedslightly, the ratio of TXB₂/6-keto-PGF₁α was stabilized. It was suggested that TMP has definitetherapeutic effects on AHNP-related lung injury in rats, and exerted by scavenging oxygen freeradical, inhibiting synthesis of TXA₂, augmenting production of PGI₂, and maintaining balancebetween TXA₂ and PGI₂.

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