肝硬变患者血清腺苷脱氨酶和外周血T细胞亚群与中医证型关系

用改良Ｍａｒｔｉｎｅｋ微量测定法检测７４例肝硬变患者和１００名健康人血清腺苷脱氨酶（ＡＤＡ）含量，用间接免疫荧光法检测３８例肝硬变患者和６０名健康人外周血Ｔ淋巴细胞亚群，并探讨其与中医证型间的关系。结果显示：肝硬变患者血清ＡＤＡ显著高于健康人（Ｐ＜０．０１），并按肝郁脾虚型、热郁血瘀型、阴虚络阻型顺序依次递增，各证型间有非常显著差异（Ｐ＜０．０１）；ＯＫＴ８明显高于健康人、ＯＫＴ４／ＯＫＴ８比值则明显低于健康人（Ｐ＜０．０５～０．０１），但３个证型间无明显差异（Ｐ＞０．０５）。本研究结果表明血清ＡＤＡ似与肝硬变程度及中医证型有关，可考虑作为本病中医辩证分型、判断病情与预后的参考指标之一。

英文摘要:

Serum adenosine deaminase(ADA) of 74 liver cirrhosis patients and 100 healthy subjects as control were examined with improved Martinek microassay and peripheral T lymphocyte subsets of 38 liver cirrhosis patients and 60 healthy subjects studied by indirect immunofluorescence assay(IFA) for exploring the relationship between them and syndrome types of TCM.The result showed that level of ADA of liver cirrhosis patients was higher than that of control(P<0.01) and increased in following order: the type of Liver-energy Depression and Spleen Deficiency,that of Heat -Stagnation and Blood Stasis and that of Yin-Deficiency and Microvessel Obstruction.The difference of serum ADA among the types were significnat(P<0.01).The result also showed that OKT.of liver cirrhosis patients was higher,the ratio of OKT4/OKT8 was lower than the healthy subjects(P<0.05-0.01),but the difference among the types were not significnat(P>0.05).Serum ADA seemed to be one of the teference indexes in differentiating syndrome types of TCM,determining the patient’s condition and prognosis.

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