中药伍铜铁复合制剂对癌细胞恶性程度影响的研究

作者	单位
迟永春	中日友好医院临床医学研究所
周舒	中日友好医院临床医学研究所
万方	中日友好医院临床医学研究所
徐梅	中日友好医院临床医学研究所
杜皓萍	中日友好医院临床医学研究所

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中文摘要:

ＢＡＬＢ／ｃ小鼠荷腹水型肝癌，连续灌喂中药伍铜铁复方制剂（ＣＨＣＦ）１０天，流式细胞光度分析结果表明：ＣＨＣＦ可使癌细胞ＤＮＡ含量降低；增殖指数（ＰＩ）下降（对照组８３．４±２．６，给药组７８．８±１．５或对照组６７．２±１．３，给药组６４．２±１．６，Ｐ＜０．０５）；Ｇ１期细胞数明显增多，而Ｓ＋Ｇ２Ｍ期细胞数明显减少（Ｐ＜０．０５～０．０１）；ＤＮＡ组方图中二倍体峰明显增高变大，而多倍体峰变小。再移植实验证明：经ＣＨＣＦ处理过的癌细胞再移植的动物有２／１０不长肿瘤，肿瘤抑制率为７１．７％～８８．３％；再移植动物存活期明显延长，从２６．１±１１．８天延长至３８．１±９．６天或延长至３９．６±７．２天，生命延长率（Ｔ／Ｃ）分别为４６％和５２％。因而证明：该制剂降低了小鼠肝癌细胞的恶性程度，其作用机理可能是激活癌细胞膜的氧化磷酸化过程。

英文摘要:

BALB/c mice bearing ascitic liver cancer were used,and Chinese herbal prescriptions combined with Cu and Fe(CHCF) was given by gavage continuously for 10 days,and some cell biological parameters were measured; furthermore,the ascitic cancer cells(control and treated group) were removed,and retransplanted to another mice and observed.The results showed that in CHCF treated group,DNA content of the cancer cells was decreased,and the proliferation index(PI) was reduced(control:83.4±2.6,CHCF group:78.8±1.5;or control:67.2±l.3,CHCF group:64.2±1.6,P<0.05).The number of the cancer cells in G1 phase increased obviously,but,those of S+G2M phases decreased considerably (P<0.05～0.01);on the DNA histogram,the diploid peak became higher and bigger,but multiploid peaks became smaller. Furthermore,retransplanted experiments showed that in 2/10 animals,the tumor did not grow,the growth inhibition rate was 71.7%～88.3%;and the survival period of retransplanted animals was prolonged significantly (from 26.1±11.8 days to 38.1±9.6 days,or to 39.6±7.2 days,P<0.01);the increase in life span was 46%～52% respectively.These results suggested that CHCF could decrease the malignancy of liver cancer cells in mice.

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