林秀珍,马德禄,崔荣芬.大黄素及番泻甙和大黄多糖对培养大鼠肝细胞内游离钙浓度的影响[J].中国中西医结合杂志,1995,(7):419-421 |
大黄素及番泻甙和大黄多糖对培养大鼠肝细胞内游离钙浓度的影响 |
Effects of Emodin, Sennosides and Rheum Polysaccharides on Free Calcium in Isolated Rat Liver Cells |
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DOI: |
中文关键词: 大黄素 番泻甙 大黄多糖 大鼠肝细胞 钙离子 |
英文关键词:emodin sennosides rheum polysaccharide isolated rat liver cells calcium ion |
基金项目:国家自然科学基金 |
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中文摘要: |
应用钙荧光指示剂乙酰羟甲酯研究大黄素(EMD)、番泻甙(SEN)和大黄多糖(RPP)对大鼠肝细胞内游离钙([Ca2+]i)浓度的影响。结果表明,在静息状态下肝细胞内[Ca2+]i为131.60±37.79nmol/L。依次加入CaCl2(2mmol/L)和KCl(120mmol/L)后,肝细胞内[Ca2+]i较静息时显著增加(P<0.01)。预先经EMD(0.037mmol/L)作用的肝细胞,在静息状态或加入同上剂量的CaCl2和KCl后,细胞内[Ca2+]i均较去离子水对照组明显增加(P<0.01);相反,SEN和RPP使肝细胞内[Ca2+]i明显降低(P<0.01),且抑制效应与剂量呈负相关。从大黄不同有效成分EMD、SEN和RPP对细胞内游离钙水平的不同影响提示,大黄对肝细胞功能具有多种调节作用。 |
英文摘要: |
The effects of emodin(EMD),sennosides(SEN) and Rheum polysaccharides(RPP) on the free calcium level in the isolated rat liver cells were studied with Ca2+ level 131.60±37.79nmol/L in the liver cells.After adding CaCl2(2mmol/L) and KCl(120mmol/L) to liver cell suspension sequentially,the free Ca2+ levels were significantly elevated compared with that of the resting status(P<0.01).When the liver cells were pretreated with EMD(0.037mmol/L) for 10 min, in the resting status or using the above doses of CaCl2 and KCl,the free Ca2+ levels were significantly increased compared with that of the control groups(P<0.01).On the contrary,after administration of SEN(0.046~0.092mmol/L) and RPP(0.1~0.2 mg/ml),the free Ca2+ levels were obviously decreased compared with that of the control groups(P<0.01).Furthermore,the inhibitory effect was dose dependent.The opposite effects of the different active ingredients of rhubarb(EMD,SEN,RPP) on the free Ca2+ levels suggested that rhubarb has many kinds of regulatory function in the liver cells. |
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