| 郭棋,彭天庆,杨英珍,顾全保,赵剑星.黄芪对柯萨奇B3病毒感染培养大鼠心肌细胞Ca2＋内流及该病毒RNA复制[J].中国中西医结合杂志,1995,(8):483-485 |
| 黄芪对柯萨奇B3病毒感染培养大鼠心肌细胞Ca2＋内流及该病毒RNA复制 |
| Effect of Astragalus membranaceus on Ca￣（2+） Influx and Coxsackie Virus B3 RNA Replication in Cultured Neonatal Rat Heart Cells |
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| DOI： |
| 中文关键词: 病毒性心肌炎 柯萨奇B3病毒 黄芪 钙内流 |
| 英文关键词:Coxsackie virus B3 viral myocarditis Ca￣(2+) influx Astragalus membranaceus |
| 基金项目:国家自然科学基金 |
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| 中文摘要: |
| 本研究结果发现，黄芪对柯萨奇Ｂ３病毒（ＣＶＢ３）感染大鼠心肌细胞及正常对照心肌细胞的Ｃａ２＋内流均呈显著抑制作用（ｐ＜０．０１，Ｐ＜０．０５）)；若在病毒感染１ｈ后加黄芪，经４８ｈ培养，对正常对照及感染细胞的Ｃａ２＋内流亦有抑制作用（Ｐ＜０．０５）；同时细胞中ＣＶＢ３－ＲＮＡ含量显著少于病毒对照组（Ｐ＜０．００１）。提示黄芪具有钙拮抗作用，可减少病毒感染引起的心肌Ｃａ２＋内流量，有可能减轻感染细胞的继发性Ｃａ２＋损伤；且对细胞中病毒ＲＮＡ的复制具有抑制作用，显示了其在病毒性心肌炎临床治疗上的应用价值。 |
| 英文摘要: |
| The effect of Astrapalus membranaceus(AM) on Ca2+ influx across the myocardial plasma membrane and coxsackie virus B3(CVB3)-RNA replication in cultured neonatal rat heart cells infected with CVB3 was investigated.It was found that the Ca2+ influx could be inhibited significantly(P<0.01) by AM after infection of heart cells for 48 h.In addition,when the cultured heart cells infected with CVB3 and treated with AM for 48 h,the Ca2+ influx of infected heart cells also could be inhibited by AM(P<0.05) and the amounts of CVB3-RNA in myocytes were significantly decreased than that in infected control group(P<0.001).These phenomena suggested that AM could exert the effects of decreasing the secondary Ca2+ damages,and improving the abnormal myocardial electric activity,and inhibiting replication of CVB3-RNA in myocardium.Thus, it is a rational choice to treat patients with AM in viral myocarditis. |
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