唐利龙,汪丽惠,张均华,朱国英,张小荣.川芎嗪对原代培养血管平滑肌细胞胶原基因表达的影响[J].中国中西医结合杂志,1995,(11):666-668 |
川芎嗪对原代培养血管平滑肌细胞胶原基因表达的影响 |
Effect of Tetramethylpyrazine on Expression of Collagen Genes in Primary Cultured Smooth Muscle Cells in Rats |
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DOI: |
中文关键词: 川芎嗪 血管平滑肌细胞 胶原 |
英文关键词:collagen vascular smooth muscle cells tetramethylpyrazine |
基金项目:国家八五攻关项目 |
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中文摘要: |
为了观察川芎嗪对冠状动脉成形术(PTCA)后再狭窄的治疗作用及其活血化瘀的可能机制,我们用40μg川芎嗪来处理培养血管平滑肌细胞(VSMCs),应用Northern杂交的方法观察了川芎嗪对培养VSMCs前胶原α1(Ⅰ)、α1(Ⅲ)基因转录的影响,发现川芎嗪能明显抑制α1(Ⅰ)、α1(Ⅲ)基因的转录。 |
英文摘要: |
For the purpose of studying the mechanism of tetramethylpyrazine(TMP) to prevent the restenosis after percutaneous transluminal coronary angioplasty(PTCA) and promote blood circulation to remove stasis.40μg of TMP was used to treat cultured vascular smooth muscle cells (VSMs).The total RNA was extracted from treated VSMC.The RNA Northern blot hybridization with procollagen genesαi-Ⅰandα1-Ⅲ probes was used to investigate its change in mRNA level.The results indicated that TMP could inhibit significantly the transcription of procollagen genesα1-Ⅰ andα1-Ⅲ. |
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