猪苓多糖、香菇多糖和分枝杆菌多糖对淋巴因子活化杀伤细胞活性增强作用的研究

Study on the Enhancing Effect of Polyporus Polysaccharide,Mycobacterium Polysaccharide and Lentinan on Lymphokine-Activated Killer Cell Activity in vitro

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DOI：

中文关键词: 淋巴因子活化的杀伤细胞白细胞介素－2 多糖

英文关键词:lymphokine-activated killer cell interleukin-2 polysaccharide

基金项目:

作者	单位
李金锋	唐山市人民医院
郭静文	唐山市人民医院
黄信孚	唐山市人民医院
林本耀	唐山市人民医院

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中文摘要:

本实验对猪苓多糖（ＰＰＳ）、香菇多糖（ＬＥＮ）和分枝杆菌多糖（ＭＰＳ）在体外对淋巴因子活化杀伤（ＬＡＫ）细胞活性的影响进行了研究，将三种不同浓度的多糖与不同浓度的重组白细胞介素２（ｒＩＬ－２）一起共同培养健康人外周血单个核细胞（ＰＢＭｃ），９６小时后以氚－胸腺嘧啶核苷（３Ｈ－ＴｄＲ）掺入法细胞毒试验检测ＬＡＫ细胞对不同靶细胞（包括ＮＫ敏感和ＮＫ抵抗）的体外杀伤活性。结果发现：三种多糖在一定浓度范围内与ｒＩＬ－２合用能增加ＬＡＫ细胞活性４２％～５６．９％，降低ｒＩＬ－２用量５０％（Ｐ＜０．０５～０．０１）。结果提示：三种多糖均可作为细胞活性上向调节剂（ＬＵＲＡ）用于ＬＡＫ细胞疗法。

英文摘要:

The actions of Polyporus polysaccharide(PPS),mycobacterium polysaccharide(MPS) and lentinan (LEN) on lymphokine-activated killer(LAK) cell activity in vitro were investigated in this study.Human peripheral blood mononuclear cells(PBMC) were cultured for 96 hours with medium containing different concentrations of the above-mentioned drugs in combination with recombinant interleukin 2(rIL-2).Then cell-mediated lysis was determined by 3H-TdR release assay including NK sensitive and NK resistant target cells.The results demonstrated that,when combined with rIL -2 in a certain concentration,all three kinds of polysaccharides could enhance the LAK activity by 42%～56.9%,and reduce the dose of rIL-2 by 50%(p<0.05～0.01).It suggested that the PPS,MPS and LEN could be used as bioactivity regulators in LAK cell therapy in tumor treatment.

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