栀子对急性胰腺炎大鼠胰腺细胞亚细胞器的保护作用

本实验利用去氧胆酸钠胰管内注射诱发大鼠急性胰腺炎（ＡＰ），以琥珀酸脱氢酶（ＳＤＨ）、酸性磷酸酶（ＡＣＰ）、细胞色素Ｐ４５０分别作为线粒体、溶酶体和微粒体的标志酶，检测ＡＰ时胰腺细胞亚细胞器的功能变化及栀子的影响，并就组织学的变化作了同步观察。结果显示：ＡＰ动物胰腺细胞内ＳＤＨ明显降低，ＡＣＰ释放率显著升高，栀子治疗组ＳＤＨ与正常接近，ＡＣＰ释放率明显降低，Ｐ４５０增高。组织学检查表明ＡＰ动物的胰腺组织有明显的炎症改变，亚细胞器结构异常；用栀子治疗者，病变明显减轻。提示栀子对ＡＰ时胰腺细胞亚细胞器的功能、结构均有良好的保护作用。

英文摘要:

The acute pancreatitis(AP) was induced with sodium deoxy-cholate in SD rats.Succinic dehydrogenase(SDH),acid phosphatase(ACP) and cytochrome P450 were taken as marked enzyme in estimation of mitochondria,lysosome and microsome to observe the functional and structural changes of the pancreatic subcellular organs and the protective effects of Fructus Gardeniae(FG)in acute pancreatitis in rats.The histological change was observed simultaneously.The results showed that pancreatic cellular SDH reduced significantly and the releasing rate of ACP raisedobviously in AP,in FG treated group,SDH was nearly the same as in normal,the releasing rate of ACP decreased and the content of P450 increased in the same time.Histologic observation showed that there was significant inflammatory change in pancreas and abnormal manifestation in structure of subcellular organs in AP.FG Could alleviate the lesion. These results suggested the FG has a remarkable protective effect on the function and structure of the pancreatic subcellular organs in AP.

关闭