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白坚石,何忠效,吴志奎,崔京华.抗衰老药物对大鼠脑DNA甲基化酶的影响[J].中国中西医结合杂志,1996,(6):358-359
抗衰老药物对大鼠脑DNA甲基化酶的影响
Effect of Anti-Aging Drug on DNA Methylase of Brain in Rats
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DOI:
中文关键词:  神方补肾生血药  抗衰老  DNA甲基化酶
英文关键词:Shenfang Bushen Shengxue Drug  anti-aging  DNA methylase
基金项目:国家自然科学基金
作者单位
白坚石  
何忠效  
吴志奎  
崔京华  
摘要点击次数: 1129
全文下载次数: 838
中文摘要:
      以 ̄3H-甲基掺入法测定大鼠脑中DNA甲基化酶活性,发现在长期服用神方补肾生血药后,大鼠脑DNA甲基化酶比活力增高,酶的热稳定性和对NaCl的耐受力略有增强,最适pH改变。用SDS-PAGE检测酶粗提物的蛋白成分,发现给药组电泳行为有所改变。说明神方补肾生血药改变大鼠脑DNA甲基化酶活性和酶学性质,从而影响其DNA甲基化水平,可能是该药物抗衰老作用的途径之一。
英文摘要:
      DNA methylase activity of brain in Wistar rats treated with Shenfang Bushen Shengxue Drug(SBSD) was observed using 3H-labelled methyl group of S-adenosyl-methionine incorporated into DNA.It was found that the SBSD has a marked effect on anti-aging. After SBSD treatment the specific activity of DNA methylase increased, the thermostability and salt-tolerance of it improved slightly,its optional PH changed from 7.5 to 8.0.A and a difference was found between the electrophoresgram of partially purified product of DNA methylase in SBSD treated rats and that in normal rats,suggesting SBSD could change the activity and characteristics of DNA methylase so as to affect the level of DNA methylation,which might be one pathway of SBSD effects on anti-aging.
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