| 冯前进,冯玛莉,王玉良,杨艳华,武玉鹏.补肾方剂左归丸（汤）对小鼠早期胚胎发育的影响[J].中国中西医结合杂志,1996,(11):673-675 |
| 补肾方剂左归丸（汤）对小鼠早期胚胎发育的影响 |
| Effect of Zuoguiwan on Early Embryonic Development of Mice |
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| DOI： |
| 中文关键词: 左归丸 早期胚胎发育 体外培养 |
| 英文关键词:Zuoguiwan early embryonic developmental,culture in vitro |
| 基金项目:国家中医药管理局科研基金 |
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| 摘要点击次数: 1317 |
| 全文下载次数: 1164 |
| 中文摘要: |
| 本研究通过体外用药、体外用给药小鼠血清及在体用药三种方式，以酒精制成胚胎发育迟缓模型，以气血双补方八珍汤作对照，应用补肾阴方左归丸（汤），对体外培养的小鼠２细胞期（２ＣＥ）至囊胚期胚胎发育进行了动态观察。结果表明：用给药小鼠血清或在体应用左归汤者，体外培养的２ＣＥ至囊胚各期胚胎的发育率均显著高于酒精模型组，八珍汤组各期胚胎发育率略高于模型组，但无显著性差异；体外用药对早期胚胎发育未见明显作用。 |
| 英文摘要: |
| Effects of Zuoguiwan (ZGW,a prescription for reinforcing Kidney Yin) on early embryonic development were observed by using embryonic developmental retardation model of mice formed by alcohol. Drug was given in three ways:add ZGW into cultural medium directly(group A),add the serum of mice received ZGW(group B) and cultured the embryo taken from ZGW treated mice (group C).The result was compared with that treated with Bazhen decoction (BZD, a prescription for supplementing Qi and blood). Results showed that the in vitro developmental rate of embryo from 2-cell stage to blastula stage in group B and C, which approached to normal control group, was higher than that in untreated model obviously.While in BZW group, it was higher than in normal control group only in certain stage. However, adding ZGW directly into culture medium didn’t reveal marked effect on early embryonic development. |
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