菟丝子、仙茅、巴戟天对人精子体外运动和膜功能影响的研究

目的：观察菟丝子、仙茅、巴戟天对人精子体外运动和膜功能的影响。方法：取不同浓度的菟丝子、仙茅、巴戟天水煎剂与人体精子混匀，３７℃孵育３０ｍｉｎ，通过精子毛细管穿透试验，精子运动速度和活力指数计算评估精子活动功能，采用精子低渗肿胀试验评价其膜功能。结果：精子的运动功能、膜功能均显著改善，其中以菟丝子最佳，仙茅次之，巴戟天则较差。结论；菟丝子对精子的运动能力和膜功能有促进作用。故有益于治疗男性不育并可能有助于提高人工授精的成功率。

英文摘要:

To observe the effects of Semen Cuscutae (SC),Rhizoma Curculiginis(RC)and Radix Morindae Officinalis(RMO) on human sperm motility in vitro and cytomembrane function. Methods:Compare the sperm motility of the above-mentioned medicinal plants with modified Tyrode’s solution (MTS)and MTS added caffeine in vitro. Human spermatozoa were incubated with decoctions of medicinal plant at various concentrations in 37℃ for 30 minutes. The motility of sperm was evaluated by the sperm capillary penetrating test,sperm speed test and sperm activity index calculations.The spermatozoa membrane function was evaluated by spermatozoal hypo-osmotic swelling test together with eosin staining methods. Results:The sperm motility improved markedly and sperm membrane function became more stabilized after incubation, the SC had best effect, RC took second place,RMO was relatively poor. Conclusions:SC decoction is an effective promoting preparation for sperm motility and the membrane function stabilized. Therefore, SC may be beneficial in treatingmale sterility and may raise success rate in artificial insemination.

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