复方白龙片对人胃癌细胞两条信号通路的调控

目的：观察复方白龙片（白龙），对人胃癌细胞内两条信号通路共调作用。方法：分别测定不同状况下人胃癌ＭＧｃ８０－３细胞内ｃＡＭＰ水平、二酰基甘油（ＤＡＧ）含量、蛋白激酶Ａ（ＰＫＡ）和蛋白激酶Ｃ（ＰＫＣ）活性。结果：白龙处理ＭＧｃ８０－３细胞３ｈ后，ｃＡＭＰ水平和ＰＫＡ活性分别较对照组升高１１２．７％和１９．７％，而ＤＡＧ含量与ＰＫＣ活性分别下降４７．０％和６４．２％；如用ＧＦ－１０９２０３Ｘ阻断ＰＫＣ通路时发现ｃＡＭＰ水平与ＰＫＡ活性分别升高７８．９％和３３．５％，而ＤＡＧ含量与ＰＫＣ活性则降低４０．３％和５６．３％；若白龙处理同时加ＰＫＡ抑制剂则较白龙单纯处理时ｃＡＭＰ水平下降４６．４％，ＰＫＡ活性下降２８．９％，而ＤＡＧ含量与ＰＫＣ活性分别升高５０．７％与５４．５％。结论：（１）白龙对ＭＧｃ８０－３细胞的增殖调控和两条信号系统之间具有因果关系。（２）该结果与环六亚甲基双乙酰胺对ＭＧｃ８０－３细胞信号系统的影响完全相似，反映出信号系统对肿瘤细胞交叉共调作用可能是中西医药对肿瘤细胞调控的共同物质基础。

英文摘要:

Objective: To evaluate the effects of traditional Chinese medicine formulae (Bailong) on cAMP/PKA and diacylaglycerol (DAG) protein kinase (PKC) pathways of the MGc80-3 cells. Methods:cAMP level, DAG content and activities of PKA and PKC were measured in different groups:(1) control, (2) 1. 8 mg/ml Bailong, (3) 1. 8 mg/ml Bailong +20 μg/ml PKA inhibitor,(4) 5 μmol/L PKC inhibitor. Results: When MGc80-3 cells were in treatment with Bailong for 3 hours, cAMP level and PKA activity were 11 .27%, and 19. 7% higher than that of the control, while DAG content and PKC activity were 47. 0% and 64. 2% lower than that of the control; When PKC pathway was blocked by PKC inhibitor GF-109203X, cAMP level and PKA activity were increased by 78. 8 % and 33. 5 % as against that of the control,while the DAG content and PKC activity were decreased by 40.3% and 56.3% respectively.When MGc80-3cells were treated with Bailong, and at the same time, blocked PKA pathways by PKA inhibitor, cAMP level and PKA activity were decreased by 46.0% and 28.9%, on the other hand, DAG content and PKC activity were increased by 50. 7 % and 51.6% as against that of the Bailong group.Conclusions: (1) There was relationship of causes and result between differentiation of MGc80-3 cells and the signal pathways. (2)Results of this study were similar to that of hexamethylenebisacetamide de (HMBA). It is shown that the two signal systems are the foundation of regulative effects of Chinese medicine formulae Bailong or HMBA on proliferation and differentiation in MGc80-3 cells.

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