左萍萍,刘娜,雒蓬轶,沈琦,唐利军,胡韶毅.还少丹的脑保护机制研究[J].中国中西医结合杂志,1997,(7):420-422 |
还少丹的脑保护机制研究 |
Protection Mechanism of Huanshaodan Decoction in Brain |
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DOI: |
中文关键词: 还少丹 脑损伤 学习记忆 N-甲基-D-天门冬氨酸受体 胆碱乙酰化转移酶 |
英文关键词:Huanshaodan brain injury N-methyl-D-Aspartic acid receptor learning and memory choline acetyltransferase |
基金项目:国家自然科学基金 |
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中文摘要: |
目的:为探讨中药古成方“还少丹”的脑保护机制,观察了该药对脑兴奋性损伤模型的预防及治疗作用。方法:利用小鼠双侧颈总动脉结扎及反复再灌的手术造成小鼠智力下降(避暗实验次数增多)的脑损伤模型,从行为学方面观察了还少丹对脑损伤小鼠学习记忆能力的改善作用,并进一步利用受体研究方法考察了该药的脑保护机制。结果:还少丹可明显减少术后小鼠避暗实验的错误次数(模型组为2.00±0.34次/5min,单纯治疗组为0.75±0.25次/5min,P<0.01;预防加治疗组为0.38±0.18次/5min,P<0.01)。受体结合实验表明,还少丹明显抑制小鼠脑损伤后引起的大脑皮层、海马两部位N-甲基-D-天冬氨酸(即NMDA)受体的激活,与模型组比较,有明显差异(P均<0.01);同时发现用药后胆碱乙酸化转移酶(CAT)的活性明显提高(P<0.01)。结论:揭示该药具有对抗兴奋性氨基酸毒性的作用。还少丹还可明显增强脑损伤模型的皮层、海马部位的CAT活性。 |
英文摘要: |
Objective:To observe the protective effect of Huanshaodan(HSD) in mouse brain in a steady ischemia-reperfusion model. Methods: Step-through experiment, receptor binding test and choline acetyl-transferase (CAT) activity detemination was performed. Results: After HSD treatment, mice made much less mistakes in step-through experiment than sham grouPS. Meanwhile the 3H-MK801 binding of cerebral cortex and hippocampus tissues was significantly decreased. CAT activity Of cerebral cortex and hippocampus tissue were increased respectively. COnclusion: HSD might antagonize ischemia injury of brain through modulating excessive activation of glutamate N-methyl-D-Aspartic acid receptors. |
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