张国强,叶任高,孔庆瑜,李幼姬,关伟明.丹参对狼疮性肾炎成纤维细胞增殖、凋亡及c-myc蛋白表达的影响[J].中国中西医结合杂志,1997,(8):473-475 |
丹参对狼疮性肾炎成纤维细胞增殖、凋亡及c-myc蛋白表达的影响 |
Effects of Radix Salivae Miltiorrhizae on Proliferation, Apoptosis and c-myc Protein Expression of Fibroblast in Culture of Kidney with Lupus Nephritis |
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DOI: |
中文关键词: 丹参 成纤维细胞 肾间质 细胞凋亡 细胞增殖 c-myc蛋白 |
英文关键词:Radix Salviae Miltiorrhizae fibroblast renal interstitial tissue apoptosis proliferation c-mycprotein |
基金项目:国家自然科学基金 |
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中文摘要: |
观察丹参对人肾间质成纤维细胞生物学行为的影响。方法:用狼疮性肾炎(LN)患者肾活检组织培养分离的成纤维细胞,观察丹参对人肾成纤维细胞3H-TdR掺入率的影响,并用流式细胞仪检测了细胞凋亡及c-myc蛋白表达情况。结果:丹参对人肾成纤维细胞增殖有抑制作用,并通过使c-myc蛋白高水平表达而诱导细胞凋亡。结论:患者长期使用大剂量丹参治疗,可能对LN的间质纤维化病变有一定疗效,从而防止或减少疤痕的形成,延缓尿毒症的发生。 |
英文摘要: |
To observe the effects of Radix Salviae Miltiorrhizae (SM) on human fibroblast in culture of kidney with lupus nephritis (LN). Methods: Fibroblasts was isolated from culture of kidney biopsy of LN patients, and effect of SM on 3H-TdR incorporated rate of fibroblasts was observed. The apoptosis and c-myc expression were detected in the same time by flow cytometry. Results: SM could inhibit the proliferation of fibroblast, and promote the programmed cell death through upregulate c-myc protein expression in human renal fibroblasts. Conclusions:Long-term administration of SM in large dosage could be effective on interstitial fibrosis of LN, so that to prevent or reduce the scar tissue formation and retard the occurrence of uremia. |
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