济肾汤对糖尿病大鼠肾脏病变的影响

目的：观察济肾汤对实验性糖尿病大鼠肾脏病变的改善作用。方法：将糖尿病大鼠于４周后再随机分为３组。糖尿病组，糖尿病依那普利治疗组（依那普利用量为每日１ｍｇ／ｋｇ，作为阳性对照药）和糖尿病济肾汤治疗组（济肾汤用量为每日２２．４ｇ／ｋｇ），灌胃给药治疗８周。观察治疗前后肾脏变化，血清肌酐（ＳＣｒ）尿素氮（ＢＵＮ），血脂，尿微量白蛋白（Ａｌｂ）、β２－微球蛋白（β２－ｍ）、Ｎ－乙酰－β－Ｄ氨基葡萄糖苷酶（ＮＡＧ）活性，血脂变化。结果：济肾汤可减轻糖尿病大鼠肾脏肥大和降低肾重／体重比值（Ｐ＜０．０１），使糖尿病大鼠血ＳＣｒ、ＢＵＮ、尿Ａｌｂ和β２－ｍ含量和ＮＡＧ活性明显降低（Ｐ＜０．０１），具有良好降低甘油三酯、胆固醇和升高高密度脂蛋白－Ｃ水平的作用（Ｐ＜０．０１），亦减少了糖尿病大鼠肾小球基底膜厚度和系膜ＰＡＳ染色区体密度。结论：济肾汤对糖尿病大鼠肾脏功能和结构有明显改善作用。

英文摘要:

To observe the ameliorative effect of Jishentang (JST) on renal lesions in experimental diabetic rats. Methods: The diabetic rats were randomly divided into three groups: group with out treatment, group with enalapril treatment (the dose of enalapril was 1 mg/kg·d) and group with JST treatment (the dose of JST was 22. 4 g/kg·d). The treatment lasted for 8 weeks. Results: (1)JST treatment attenuated the renal hypertrophy and ratio of kidney and body weight(P<0. 01 ). (2) JST treatment reduced the levels of SCr and BUN and the levels of Alb, β2-m and the activity of NAG in urine in diabetic rats significantly (P<0. 01). The levels of TG and TCh decreased and the levels of HDL-C increased in the serum of the JST treated diabetic rats (P<0. 01). (3 ) The glomerular basement membrane thickening and volume density of PAS positive staining in mesangial area were significantly decreased in JST treated diabetic rats. Conclusions: JST exerted obvious ameliorative effect on renal function and structure in diabetic rats.

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