吕爱平,刘振丽,张枢明,王安民.宣发膜原法对免疫性关节炎(痹证)小鼠免疫功能的影响[J].中国中西医结合杂志,1997,(11):679-681 |
宣发膜原法对免疫性关节炎(痹证)小鼠免疫功能的影响 |
Effect of Immune Function Xuanfa Moyuan Principle on Immune Arthritis (Bizhen) in Rats |
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DOI: |
中文关键词: Ⅱ型胶原 免疫 关节炎 宣发膜原法 痹证 |
英文关键词:type Ⅱ collagen immune arthritis Xuanfa Moyuan prineiple Bizheng |
基金项目:国家自然科学基金 |
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中文摘要: |
目的:探讨宣发膜原法对免疫性关节炎(痹证)小鼠免疫功能的影响。方法:运用Ⅱ型胶原免疫所致小鼠关节炎作为中医痹证动物模型,用过原饮作为宣发膜原法的代表方,对痹证小鼠给药前后关节炎发病程度,血清抗Ⅱ型胶原抗体、IgM型类风湿性因子、淋巴结细胞产生白细胞介素10(IL-10)和γ-干扰素(IFN-γ)分别用记分法和免疫酶标法进行了检测。结果:宣发膜原法能推迟小鼠关节炎的发病时间,降低发病率,降低小鼠血清抗Ⅱ型胶原抗体IgG2a、IgG2b和IgG1水平和IgG2a+IgG2b与IgG1的比值,降低淋巴细胞产生IFN-γ的水平,提高其产生IL-10的能力。结论:宣发膜原法不仅能抑制小鼠对Ⅱ型胶原的免疫反应,同时能调节小鼠对Ⅱ型胶原的免疫反应。 |
英文摘要: |
To explore the effect of Xuanfa Moyuan principle (XFMYP) on arthritic mice. Methods: The type Ⅱ collagen induced arthritis in DBA mice was taken as Bizheng animal model. The Dayuanyin (DYY) was taken as the representative prescription for XFMYP. The arthritis in-cidence and arthritis index were tested by scoring system. The anti-C Ⅱ antibody IgG and its subsets, IgM rheumatoid factor, interferon gamma and interleukin 10 produced by lymph node cells were tested by ELISA method. Results: XFMYP could delay the onset day of arthritis, decrease the arthritic incidence and index, decrease anti-Ⅱ IgG2a, IgG2b,IgG1 level and the IgG2a + IgG2b/IgG1 ratio in serum, decrease the production of interferon gamma by lymphocytes and increase the production of interleukin 10. Conclusions: XFMYP could inhibit the anti-C Ⅱimmune response, and balance the anti-C Ⅱ Th1 and Th2 type immune responses. |
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