灭囊灵对体外猪囊尾蚴作用的电镜观察

目的：观察灭囊灵在体外对猪囊尾蚴的治疗作用。方法：用扫描电镜和透射电镜观察灭囊灵醇提物、毗唾酮和丙硫咪唑体外作用于猪囊尾蚴后的病变。结果：扫描电镜下见灭囊灵和吡喹酮组头节均未翻出，囊壁萎缩，皮层外质膜受损，微毛结构不清或脱落，表面布满颗粒状物；丙硫咪唑组４６．７％已脱囊，顶突头钩发育正常，４个吸盘表面凹陷，颈部糜烂，体节正常沟条消失。透射电镜下见灭囊灵和吡喹酮组虫体皮层、基底膜、肌层和实质层均明显受损，但灭囊灵对实质层神经索的损伤作用优于吡喹酮，丙硫咪唑组虫体各层次损伤均明显轻于灭囊灵和吡喹酮。结论：中药灭囊灵对猪囊尾蚴具有明显的杀伤作用。

英文摘要:

Objective: To observe the effect of Mienangling (MNL) in treating cysticercus cellulosae in vitro. Methods:The changes of cysticercus cellulosae treated in vitro with the MNL extracts, praziquantel (PQT) and albendazole (ALB) were studied under the transmission and scanning electron microscopes. Results: Under the scnning electron microscope, the authors can see that all of the cysticercus’s scoleces did not grow in the MNL and PQTgroups, cyst walls shrivelled, tegument’s ectoplasts were damaged, microtriches’s ultrastructures were not clear or exfoliated, some corpuscular matter densely was distributed in the surface; 46. 7 % of cysticercus cellulosaes in the　ALB group had been excystated, the rostellar hooks of rostella developed normally, four sucker’s surface wrer hollow, the necks were eroded, and grooves of sonite disaPPeared. Under the transmission electron microscope, we can see that the tegument, basement membrane, muscular layer and parenchyma in the groups of MNL and PQT were all clearly damaged, but the harmful effect of MNL to parenchyma’s nerve cords is more serious than the PQT. The damage of all layers of the cysticercus cellulosae in the ALB group is smaller in degree than the MNLand the PQT. Conclusion: The MNL has marked damaging effect to cysticercus cellulosae.

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