更年健上调老年雌性大鼠下丘脑雌激素受体mRNA表达对P物质和β-内啡肽的影响

作者	单位
步世忠	Bu Shizhong, Sun Mei, Zhang Yuan, et al (Obstetric and Gynecdogy Hospital,Shanghai Medical University, Shanghai 200011
孙梅	Bu Shizhong, Sun Mei, Zhang Yuan, et al (Obstetric and Gynecdogy Hospital,Shanghai Medical University, Shanghai 200011
张沅	Bu Shizhong, Sun Mei, Zhang Yuan, et al (Obstetric and Gynecdogy Hospital,Shanghai Medical University, Shanghai 200011
张永莲	Bu Shizhong, Sun Mei, Zhang Yuan, et al (Obstetric and Gynecdogy Hospital,Shanghai Medical University, Shanghai 200011
俞瑾	Bu Shizhong, Sun Mei, Zhang Yuan, et al (Obstetric and Gynecdogy Hospital,Shanghai Medical University, Shanghai 200011

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中文摘要:

目的：探讨中药复方更年健上调老年雌性大鼠下丘脑雌激素受体（ＥＲ）和ＥＲ信使核糖核酸（ｍＲＮＡ）对Ｐ物质（ＳＰ）和β－内啡肽（β－ＥＰ）的影响。方法：建立自然衰老的雌性大鼠模型，用放射配体结合分析法检测ＥＲ，用Ｎｏｒｔｈｅｒｎｂｌｏｔ分析ＥＲｍＲＮＡ的改变，用放射免疫分析法检测ＳＰ和β－ＥＰ含量。结果：老年雌性大鼠性减退期下丘脑ＥＲ和ＥＲｍＲＮＡ水平随血清Ｅ_２水平的下降较性成熟期显著下降，而更年健则使下降了的ＥＲ和ＥＲｍＲＮＡ水平明显提高，并通过上调下丘脑ＥＲｍＲＮＡ使ＳＰ明显下降，而β－ＥＰ则显著提高，与提高体内雌激素水平具有同样的效果。结论：更年健可增加ＲＲ在下丘脑的表达以增强雌激素的生物学效应，同时降低下丘脑ＳＰ水平而提高 β－ＥＰ的含量。

英文摘要:

Objective: To investigate the effect of Chinese herbal formula Gengnianjian on estrogen receptor （ER）, ERmRNA substance P （SP） and β-endorphin （β-EP） in the hypothalamus of aging female rats. Methods: ER proteins were determined by radioligand binding assay, ER mRNA was analyzed by Northern blot analysis and the levels of β-EP and SP were detected by immunohistochemistry. Results; In aging rats, lowered ER and ER mRNA in the hypothalamus were still up-regulated by low serum E₂ level, but not in rats of after the age of 21 months; Gengnianjian significantly increased ER protein level and ER mRNA expression, decreased SP and increased the level of PEP in the hypothalamus in aging female rats before 21 months of age, without changing of serum E₂ level. In EB group, serum E₂ and β-EP levels increased and SP level decreased but ER expression suppressed in the hypothalamus except after 21 months. Conclusion: Enhance estrogen bioactivity can decrease the level of SP and increase the level of PEP in hypothalamus of aging female rats. Herbal formula Gengnianjian increase-EP level and decrease SP level by raising the level of ER and ER mRNA.

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