中药天花粉蛋白对黑色素瘤细胞凋亡及细胞周期的影响

作者	单位
毕黎琦	白求恩医科大学第三临床学院!长春　130031
李洪军	白求恩医科大学第三临床学院!长春　130031
张玉华	白求恩医科大学第三临床学院!长春　130031

摘要点击次数: 1664

全文下载次数: 1036

中文摘要:

目的：探讨中药天花粉蛋白纯化组分对黑色素瘤细胞增殖的抑制作用。方法：用流式细胞仪测定提纯的中药天花粉蛋白有效组分对小鼠黑色素瘤细胞体外培养过程中细胞ＤＮＡ合成、细胞周期及细胞凋亡的影响。结果：天花粉蛋白纯化组分可引起小鼠黑色素瘤细胞Ｇ_０／Ｇ_１期细胞增加，Ｓ期细胞减少，呈现Ｇ_０／Ｇ_１期阻滞现象。并有诱导细胞凋亡作用，而且Ｇ_０／Ｇ_１期阻滞与细胞凋亡高度相关（ｒ＝０．８７０５）。结论：天花粉蛋白纯化组分可通过抑制黑色素瘤细胞Ｓ期ＤＮＡ合成、抑制瘤细胞分裂增殖以及诱导瘤细胞凋亡来达到抑瘤作用。

英文摘要:

Objective:To study the inhibitory effect of purified trichosanthin component on the proliferation of malignant melanoma. Methods: The effect of purified trichosanthin component on the DNA synthesis, cell cycle and cell apoptosis of murine melanoma cells were detected by flowcytometry when cultured in vitro. Results: The significant G₀/ G₁ phase arrest was revealed by the increase of cells in G₀/G₁,phase and decrease of cells in S phase. The obvious apoptosis of melanoma cells was induced by purified trichosanthin component. G₀/G₁ phase arrest was highly correlated with apoptocis （r = 0 .8705 ）. Conclusions: The purified trichosanthin component can markedly inhibit melanoma cells by the suppression of DNA Synthesis in S phase and cell mitosis as well as induction of cell apoptosis.

关闭