四生汤对小鼠脾淋巴细胞DNA损伤修复功能的影响

作者	单位
凌昌全	Ling Changquan, Gao Hong, Chen Zhe, et al (Deportment of Traditional Chinese Medicine, Changhai Hospital, Shanghai 200433
高虹	Ling Changquan, Gao Hong, Chen Zhe, et al (Deportment of Traditional Chinese Medicine, Changhai Hospital, Shanghai 200433
陈喆	Ling Changquan, Gao Hong, Chen Zhe, et al (Deportment of Traditional Chinese Medicine, Changhai Hospital, Shanghai 200433
黄雪强	Ling Changquan, Gao Hong, Chen Zhe, et al (Deportment of Traditional Chinese Medicine, Changhai Hospital, Shanghai 200433
张红武	Ling Changquan, Gao Hong, Chen Zhe, et al (Deportment of Traditional Chinese Medicine, Changhai Hospital, Shanghai 200433

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中文摘要:

目的：探索四生汤减轻放射反应的作用机制。方法：用羟基脲处理培养的小鼠脾淋巴细胞，阻断Ｓ期的ＤＮＡ半保留复制，然后用紫外线照射，并加入~３Ｈ－ＴｄＲ，３７℃培养４ｈ后检测细胞~３Ｈ－ＴｄＲ掺入量，以计算出的淋巴细胞非合成期掺入指数（ＬＵＩ）为主要指标，观察四生汤对小鼠脾淋巴细胞ＤＮＡ损伤后修复功能的影响。结果：发现四生汤预处理４ｈ在体外有升高小鼠脾淋巴细胞ＬＵＩ值的作用，但加入四生汤之后立即照射紫外线的ＬＵＩ与未加四生汤组无明显差异。结论：四生汤增强小鼠脾淋巴细胞ＤＮＡ损伤修复功能的作用可能是通过与淋巴细胞共同孵育，引起ＤＮＡ修复酶系统的某些变化而实现的。

英文摘要:

Objective: To explore the mechanism of alleviating the side effects of irradiation by Sisheng Decoction (SSD). Methods: The mice splenic lymphocytes were treated by hydroxylurea to stop the DNA replication in S stage, irradiated by ultraviolet ray (UV) and the ~3H TdR was added into the cultural medium. After the lymphocytes were cultured in 37℃ for 4h, the capacity for ~3H-TdR in the lymphocytes were tested. The effect of SSD in different content on the cut and patch repair(CPR) of DNA can be inferred by observing the lymphocyte uptake index (LUI) of ~3H-TdR. Results: LUI of mice spleen lymphocytes could be increased by pretreating the lymphocytes with SSD for 4h and then irradiated by UV, but it could not be increased if the lymphocytes were treated by SSD and UV at the same time. Conclusion: SSD can alleviate the side effect of irradiation by enhancing the function of CPR-DNA, which need pretreating the lymphocytes with SSD.

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