雷公藤红素对大鼠血管平滑肌细胞c-myc和血小板源性生长因子的mRNA的影响

作者	单位
陈星	Chen Xing, Wang Luo, Feng Meifu, et al Department of Cardiology, The First Affiliated Hospital of Beijing Medical University, Beijing(100034
汪洛	Chen Xing, Wang Luo, Feng Meifu, et al Department of Cardiology, The First Affiliated Hospital of Beijing Medical University, Beijing(100034
丰美福	Chen Xing, Wang Luo, Feng Meifu, et al Department of Cardiology, The First Affiliated Hospital of Beijing Medical University, Beijing(100034
朱国英	Chen Xing, Wang Luo, Feng Meifu, et al Department of Cardiology, The First Affiliated Hospital of Beijing Medical University, Beijing(100034

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中文摘要:

目的 :观察雷公藤红素对大鼠血管平滑肌细胞 (VSMC)中原癌基因 (c myc)和血小板源性生长因子 (PDGF)的mRNA表达水平的影响。方法 :分别将所培养的第 5～ 1 0代VSMC同步化培养 2 4h后 ,加入 2 0 %胎牛血清 (FCS)和不同剂量的雷公藤红素 (0 1、0 2和0 3mg/L) ,共同孵育 6h和 1 2h后 ,分别提取VSMC胞浆总RNA ,通过地高辛标记探针 ,采用斑点杂交方法检测c myc和PDGF的mRNA表达。结果 :经雷公藤红素作用后 ,VSMC的c myc和PDGF的mRNA表达较对照组明显降低 ,c mycmRNA表达水平的降低具有一定的药物剂量依赖性关系。结论 :雷公藤红素可能是通过抑制VSMC的c myc和PDGF的mRNA的表达 ,而引起抑制VSMC的过度增殖。

英文摘要:

Objective: To observe the effect of Tripterine on mRNA expression of c myc and platelet derived growth factor (PDGF) in vascular smooth muscle cell (VSMC) of rats. Methods: 5～10th passage culture of VSMC was used. Tripterine and 20% fetal calf serum were added into the medium of cultured VSMC in concentration of 0 1,0 2 and 0 3 mg/L after free serum cultivation for 24 hours. The general RNA was isolated from VSMC at 6 and 12 hours after the drug addition for detection of mRNA expression of c myc and PDGF respectively by dot blot hybridization. The cDNA probes were labeled by digoxin. Results: Tripterine inhibited the PDGF mRNA expression of VSMC and decreased c myc mRNA expression in a dose dependent manner, either vs. control. Conclusion: Tripterine could inhibit mRNA expression of c myc and PDGF in VSMC, therefore, it would inhibit overproliferation of VSMC.

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