中西医结合治疗对慢性再生障碍性贫血患者红细胞寿命及膜蛋白的影响

作者	单位
王树庆	Wang Shuqing, Zhang Shengming, Li Jianhua, et al Affiliated Hospital of Weifang Medical College, Shandong (261031
张圣明	Wang Shuqing, Zhang Shengming, Li Jianhua, et al Affiliated Hospital of Weifang Medical College, Shandong (261031
李建华	Wang Shuqing, Zhang Shengming, Li Jianhua, et al Affiliated Hospital of Weifang Medical College, Shandong (261031
鲁崎唔	Wang Shuqing, Zhang Shengming, Li Jianhua, et al Affiliated Hospital of Weifang Medical College, Shandong (261031

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中文摘要:

目的 :探讨中西医结合治疗慢性再生障碍性贫血 (CAA)作用机理。方法 :应用51 Cr标记技术和冷冻蚀刻电镜技术对 3 0例CAA患者经中西医结合治疗前后的红细胞(RBC)寿命进行了测定 ,对其膜蛋白形态和分布进行了观察 ,并与健康人 (3 0名 )作对照。结果 :CAA患者RBC寿命缩短 ,与健康对照组比较 ,有显著性差异 (P <0 0 1 ) ,治疗后RBC寿命延长并接近健康人 ;治疗前RBC质膜内片劈裂面和外片劈裂面均见蛋白微粒形态异常、数量减少和分布不均 ,治疗后膜蛋白微粒好转 ,并接近健康人。结论 :RBC寿命与膜蛋白的形态、数量、质量及分布密切相关。中西医结合治疗CAA的疗效明显优于单纯西药疗效 ,且有稳定膜蛋白、延长RBC寿命的作用。

英文摘要:

Objective: To explore the mechanism of integrated traditional Chinese and Western medicine (TCM WM)therapy on chronic aplastic anemia (CAA). Methods: The RBC life span of 30 normal human subjects and 30 patients with CAA were measured by 51 Cr labelled technique before and after TCM WM therapy. The morphology and distribution of RBC membrane protein granules were observed by freeze fracture etching and transmission electron microscope. Results: The half life of erythrocytes (RBC T1/2)was shortened in CAA cases and there was a significant difference compared to healthy control (P<0 01). After therapy, the RBC life span prolonged and approached the normal level. Before treatment, there existed abnormal in morphology, decrease in amount and uneven in distribution of protein granules in protoplasmic face (PF) and extracellular face (EF) of RBC membrane. After treatment, the protein granules of RBC membrane was improved and approached to control. Conclusions: The morphology, amount, quality and distribution of RBC membrane protein granule were closely related to its life span. The therapeutic effect of TCM WM was better than that of WM alone and it had a function both in stabilizing membrane protein and extending the RBC life span.

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