复方丹参注射液增强白血病细胞放射敏感性作用的研究

作者	单位
谭获	Tan Huo, Ying Hongguang, Chu Jianxin, et al The Second Affiliated Hospital, Guangzhou Medical College, Guangzhou (510260
应红光	Tan Huo, Ying Hongguang, Chu Jianxin, et al The Second Affiliated Hospital, Guangzhou Medical College, Guangzhou (510260
褚建新	Tan Huo, Ying Hongguang, Chu Jianxin, et al The Second Affiliated Hospital, Guangzhou Medical College, Guangzhou (510260
郝玉书	Tan Huo, Ying Hongguang, Chu Jianxin, et al The Second Affiliated Hospital, Guangzhou Medical College, Guangzhou (510260
赵钧铭	Tan Huo, Ying Hongguang, Chu Jianxin, et al The Second Affiliated Hospital, Guangzhou Medical College, Guangzhou (510260

摘要点击次数: 1096

全文下载次数: 1021

中文摘要:

目的 :探索复方丹参注射液对白血病细胞放射敏感性的影响。方法 :采用半固体琼脂集落培养及流式细胞仪法 ,观察HL60细胞株及新鲜白血病细胞经过药物作用后 ,其放射敏感性的变化。结果 :复方丹参注射液与HL60细胞混合培养后照射 ,细胞存活曲线斜率的倒数 (D0 )、放射剂量为 2Gy时的活存比率 (SF2 )分别由 1 5 3、0 3 4降至 0 93、0 1 2 ,细胞凋亡显著增多 ;这种效应与药物浓度及作用时间存在良好的相关性 ;放射诱导的新鲜白血病细胞凋亡率与对照组 (5 89± 2 91 % )相比 ,复方丹参组 (1 2 0 5± 3 0 6% )明显增加。结论 :复方丹参注射液具有明显的放射增敏效应。

英文摘要:

Objective: To explore the effect of Composite Radix Salviae Miltiorrhizae Injection (CSMI) on radiosensitivity of leukemia cells. Methods: Semisolid agar culture and flow cytometry assay were performed for studying the change in radiosensitivity of HL60 cell line and fresh human leukemia cells after exposing to CSMI. Results: The D 0 (the inverse of the slope of the survival curve) and SF 2 (survival fraction at 2 Gy) of HL60 cell line were decreased from 1.53, 0.34 to 0.93, 0.12, respectively and apopotosis rates after radiation were raised significantly by CSMI. Furthermore, the concentration of CSMI and the time of mixed culture with CSMI before irradiation had positive relation to the effects mentioned above. Compared with control group, CSMI could increase the radiation induced apoptosis of fresh leukemia cells (5.89±2.91% vs 12.05±3.06%).Conclusion: CSMI could obviously enhance the radiosensitivity of leukemia cells.

关闭