六味地黄汤免疫调节活性成分化学分离的药理学导向评价

作者	单位
聂伟	Nie Wei, Zhang Yongxiang, Ru Xiangbin, et al Beijing Institute of Pharmacology and Toxicology, Beijing (100850
张永祥	Nie Wei, Zhang Yongxiang, Ru Xiangbin, et al Beijing Institute of Pharmacology and Toxicology, Beijing (100850
茹祥斌	Nie Wei, Zhang Yongxiang, Ru Xiangbin, et al Beijing Institute of Pharmacology and Toxicology, Beijing (100850
乔善义	Nie Wei, Zhang Yongxiang, Ru Xiangbin, et al Beijing Institute of Pharmacology and Toxicology, Beijing (100850
姚新生	Nie Wei, Zhang Yongxiang, Ru Xiangbin, et al Beijing Institute of Pharmacology and Toxicology, Beijing (100850
周金黄	Nie Wei, Zhang Yongxiang, Ru Xiangbin, et al Beijing Institute of Pharmacology and Toxicology, Beijing (100850

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中文摘要:

目的 :为定向追踪分离六味地黄汤发挥免疫调节作用的活性成分进行导向评价。方法 :运用化学与药理学密切合作的研究方法 ,以环磷酰胺 (Cy)处理小鼠为模型 ,抗体生成反应为活性评价指标 ,对每一步化学分离所得组分进行活性评价 ,确定活性部位。结果 :六味地黄汤及其醇溶、醇沉部分对Cy所致抗体生成反应低下具有明显的改善作用 ;后二者活性均高于其母体水煎剂 ;其中醇沉部分活性较醇溶部分强。醇沉部分又以活性炭柱层析所得组分CA4活性最强。结论 :六味地黄汤醇沉部分发挥免疫调节作用的主要活性成分来源于CA4,其主要由酸性多糖组成。

英文摘要:

Objective: To conduct the activity evaluation for immunomodulating components from the Liuwei Dihuang Decoction (LWDHD). Methods: Cyclophosphamide (Cy) treated mice were used as the immunodeficient model and the antibody production response in plaque forming cell (PFC) assay was employed as the activity evaluating parameter. Stepwise fractionation was guided by activity evaluation. Results: Fr1, the ethanol soluble fraction, and Fr2, the ethanol insoluble fraction of LWDHD, both significantly improved the antibody production response in Cy treated mice, in which Fr2 showed stronger activity than that of Fr1. Fr2 was further fractionated. Fr2 CA4, one of the fractions obtained by active carbon chromatography from Fr2, exhibited the strongest activity in comparison with other fractions. Conclusion: CA4, which is mainly composed of acidic polysaccharides, is the main immunomodulating active fraction contained in the ethanol insoluble fraction of LWDHD.

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