清解灵诱生的内源性集落刺激因子对增强宿主防御功能的作用

作者	单位
薛茵	Xue Yin, Pan Jufen, Ma Fengrong, et al Dept. of Immunology, Tianjin Medical University, Tianjin (300070
潘菊芬	Xue Yin, Pan Jufen, Ma Fengrong, et al Dept. of Immunology, Tianjin Medical University, Tianjin (300070
马凤蓉	Xue Yin, Pan Jufen, Ma Fengrong, et al Dept. of Immunology, Tianjin Medical University, Tianjin (300070
丁洁	Xue Yin, Pan Jufen, Ma Fengrong, et al Dept. of Immunology, Tianjin Medical University, Tianjin (300070
吴咸中	Xue Yin, Pan Jufen, Ma Fengrong, et al Dept. of Immunology, Tianjin Medical University, Tianjin (300070

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中文摘要:

目的 :为探讨清解灵 (QJL)诱生的内源性集落刺激因子 (G -CSF)对增强宿主防御功能的作用。方法 :给正常小鼠注射清解灵 ,获取血清 ,用ELISA、骨髓细胞增殖及集落生成试验证明该血清含有G -CSF活性后 ,将该血清被动转输给 8只正常BALB/C小鼠 (实验组 ) ,48h后用大肠杆菌攻击该鼠 ,次日杀鼠 ,测脾悬液中大肠菌数。对照组 (8只 )以正常小鼠血清取代实验组用的血清 ,而后同法处理。结果 :经清解灵注射的小鼠血清与抗G -CSF单克隆抗体呈阳性反应 ,该血清可促进同系鼠的骨髓细胞增殖 ,并使之在固体培养基中形成以中性粒细胞为主的细胞集落。说明该血清确实含有G -CSF。被动转输此血清的实验组小鼠 ,在受大肠杆菌攻击之后其脾悬液中的大肠杆菌数量比对照组显著减少 (P <0 0 1 )。结论 :清解灵可增强宿主的抗感染能力 ,其部分机理可能与诱导机体产生内源性G -CSF有关。

英文摘要:

Objective: To explore the effect of Qingjieling (QJL) induced endogenous granulocyte colony stimulating factor (G-CSF) in strengthening host defence against bacteria in mice. Methods: Serum containing G-CSF was obtained by injecting 5 mg/g of QJL to mice. The CSF activity was identified by ELISA, bone marrow cell proliferation and colony forming assays. Forty eight hours after the serum was infused to 8 normal BALB/C mice, the mice was sacrificed and the number of E. coli in spleen suspension was measured. Eight mice of the control group were treated in the same way but with normal serum of mice instead of the QJL treated serum. Results: The QJL treated serum showed a positive reaction with anti G CSF McAb, it promoted the proliferation of bone marrow cells and formation of cell colony of neutrophilic granulocyte type in the culture medium, these results indicated that the serum contained G-CSF undoubtedly. The number of E. coli in spleen suspension of test group was significantly lower than that of the control group P<0 01). Conclusions: QJL does enhance the defense capability of the host, the mechanism of which partly might be due to the induction of endogenous G-CSF in the host.

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