不同剂量的人参水提液皮下注射对大鼠慢性绿脓杆菌性肺炎模型的影响

作者	单位
宋志军	Song Zhijun, Johansen HK, Faber V, et al Department of Clinical Microbiology and Department of Communicable and Tropical Diseases, The National University Hospital, Copenhagen, Denmark
JohansenHK	Song Zhijun, Johansen HK, Faber V, et al Department of Clinical Microbiology and Department of Communicable and Tropical Diseases, The National University Hospital, Copenhagen, Denmark
FaberV	Song Zhijun, Johansen HK, Faber V, et al Department of Clinical Microbiology and Department of Communicable and Tropical Diseases, The National University Hospital, Copenhagen, Denmark
MoserC	Song Zhijun, Johansen HK, Faber V, et al Department of Clinical Microbiology and Department of Communicable and Tropical Diseases, The National University Hospital, Copenhagen, Denmark
吴红	Song Zhijun, Johansen HK, Faber V, et al Department of Clinical Microbiology and Department of Communicable and Tropical Diseases, The National University Hospital, Copenhagen, Denmark
KharazmiA	Song Zhijun, Johansen HK, Faber V, et al Department of Clinical Microbiology and Department of Communicable and Tropical Diseases, The National University Hospital, Copenhagen, Denmark
HoibyN	Song Zhijun, Johansen HK, Faber V, et al Department of Clinical Microbiology and Department of Communicable and Tropical Diseases, The National University Hospital, Copenhagen, Denmark

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中文摘要:

目的 :探讨人参水提液 (简称人参液 )对大鼠慢性绿脓杆菌 (PA)性肺炎模型的影响。方法 :采用 4种不同剂量的人参液皮下注射 ,观察用药两周后的疗效。结果 :4种剂量的人参液均可显著减少肺部大体观病变和肺脓肿率 (P <0 0 5) ;镜下观其病变程度与人参剂量呈负相关 (r=-0 95,P <0 0 1 ) ;人参Ⅰ (5mg/kg)、Ⅱ (2 5mg/kg)、Ⅳ (0 6 2 5mg/kg)组的肥大细胞数低于对照组 (P <0 0 5) ;Ⅱ、Ⅲ (1 2 5mg/kg)组的肺部细菌清除作用强于对照组 (P <0 0 5) ;Ⅱ、Ⅲ、Ⅳ组的PA特异性抗体应答发生下调 (P <0 0 1 ) ;Ⅰ组的IgM水平低于对照组(P <0 0 1 ) ,且IgM水平的高低与人参剂量呈正相关 (r =0 93,P <0 0 5) ;Ⅰ组的IgG2a水平高于对照组 (P <0 0 5) ;血清IgG2a水平与人参剂量呈正相关 (r =0 90 ,P <0 0 2 ) ;PA感染两周后 ,Ⅰ、Ⅱ、Ⅲ组的抗藻酸盐IgG水平高于对照组和Ⅳ组 (P <0 0 5) ,而Ⅰ、Ⅲ、Ⅳ组的IgA则低于对照组 (P <0 0 5)。结论 :2 5mg/kg的人参是本研究的最佳剂量 ,并显示了兴奋T辅助细胞Ⅰ型反应的效果

英文摘要:

Objective: To observe the effect of ginseng extracts on a rat model of chronic Pseudomonas aeruginosa (PA) pneumonia. Methods: Subcutaneous injection with four different doses of ginseng aqueous extracts to the model rats for two weeks. Results: Milder macroscopic lung pathology and lower incidence of lung abscesses were found in all the four groups received different doses of ginseng treatment compared to the control group (P<0 05). Histopathology of the lungs was negatively correlated with the ginseng doses (r=-0 95,P<0 01). The mast cell number in the lung foci in group Ⅰ, Ⅱ and Ⅳ was significantly lower than that in the control group (P<0 05). Bacterial clearance in group Ⅱ and group Ⅲ was enhanced significantly (P<0 05) compared with that in the control group. The specific antibody responses were down regulated in group Ⅱ, Ⅲ and Ⅳ (P<0 01), and IgM level in group Ⅰ was lower compared to the control group (P<0 01). Serum IgM level was positively correlated with the ginseng doses (r=0 93,P<0 05). IgG2a titer in group Ⅰ was higher than that in the control group (P<0 05). Serum IgG2a level was positively correlated with the ginseng doses (r=0 90,P<0 02). Two weeks after PA lung infection, the serum level of IgG against PA, aeruginosa alginate in group Ⅰ, Ⅱ, and Ⅲ was higher than that in the control and group Ⅳ (P<0 05), and the anti alginate IgA level in group Ⅰ, Ⅲ and Ⅳ was lower than that in the control (P<0 05). Conclusions: The ginseng dose of 2.5 mg/kg was the best dosage in the present study, and it showed an effect of stimulating T helper type Ⅰ response.

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