大蒜精油抑制白细胞介素-1α诱导的单核细胞-内皮细胞粘附及机理探讨

作者	单位
葛璐璐	第一军医大学组织胚胎学教研室
张薇	第一军医大学组织胚胎学教研室
戴云	第一军医大学组织胚胎学教研室
臧燕	第一军医大学组织胚胎学教研室
黄纯洁	第一军医大学组织胚胎学教研室

摘要点击次数: 1463

全文下载次数: 1059

中文摘要:

目的：观察大蒜精油（ＥＧＯ）对白细胞介素－１α（ＩＬ－１α）诱导的人脐静脉内皮细胞（ＨＵＶＥＣ）上血管细胞粘附分子－１（ＶＣＡＭ－１）表达及单核细胞粘附率的影响。方法：胰酶消化法分离ＨＵＶＥＣ，用Ｕ９３７单核细胞与之联合培养，分别加入ＩＬ－１α和ＥＧＯ加ＩＬ－１α孵育，计算单核细胞－内皮细胞粘附率，ＶＣＡＭ－１免疫荧光染色，粘附细胞仪（ＡＣＡＳ５７０）测定平均荧光值。结果：单独培养及联合培养的情况下，ＥＧＯ均对ＩＬ－１α诱导的ＨＵＶＥＣ上ＶＣＡＭ－１表达具有明显的抑制作用，并能显著下调ＩＬ－１α作用下单核细胞内皮细胞的粘附。结论：ＥＧＯ具有抗单核细胞与血管内皮细胞粘附的作用，其机理可能是通过抑制内皮细胞表面粘附分子的表达。

英文摘要:

Objective: To observe the effects of essential garlic oil (EGO) on vascular cell adhesive molecule 1 (VCAM 1) expression of endothelial cell and monocyte adhesion induced by interleukin-1α (IL-1α). Methods: Human umbilical vein endothelial cells (HUVEC) were isolated by pancreatin digestion method and co cultured with U937 monocyte and IL-1α or EGO+IL-1α respectively. The adhesion rate of monocyte to endothelial cell was measured and mean fluorescent intensity of immuno stained of VCAM 1 was determined by ACAS 570. Results: EGO showed significant inhibition on VCAM 1 expression induced by IL-1α either in simple culture with HUVEC or in co culture with HUVEC+U937, and could obviously down regulate the monocyte endothelial cell adhesion under effect of IL-1α. Conclusion: EGO has the effect of antagonizing against adhesion of monocyte and vascular endothelial cell, it may be due to the inhibition of EGO on adhesive molecular expression on endothelial cell.

关闭