通痹灵对大鼠佐剂性关节炎滑膜细胞功能的影响

作者	单位
陈纪藩	Chen Jifan, Zhao Huifang, Shen Xiaoyan, et al The First Affiliated Hospital, Guangzhou TCM University, Guangzhou (510405
赵会芳	Chen Jifan, Zhao Huifang, Shen Xiaoyan, et al The First Affiliated Hospital, Guangzhou TCM University, Guangzhou (510405
沈晓燕	Chen Jifan, Zhao Huifang, Shen Xiaoyan, et al The First Affiliated Hospital, Guangzhou TCM University, Guangzhou (510405
李颂华	Chen Jifan, Zhao Huifang, Shen Xiaoyan, et al The First Affiliated Hospital, Guangzhou TCM University, Guangzhou (510405
李迎敏	Chen Jifan, Zhao Huifang, Shen Xiaoyan, et al The First Affiliated Hospital, Guangzhou TCM University, Guangzhou (510405
廖世煌	Chen Jifan, Zhao Huifang, Shen Xiaoyan, et al The First Affiliated Hospital, Guangzhou TCM University, Guangzhou (510405
黄仰模	Chen Jifan, Zhao Huifang, Shen Xiaoyan, et al The First Affiliated Hospital, Guangzhou TCM University, Guangzhou (510405
奥西秀树	Chen Jifan, Zhao Huifang, Shen Xiaoyan, et al The First Affiliated Hospital, Guangzhou TCM University, Guangzhou (510405
邱联群	Chen Jifan, Zhao Huifang, Shen Xiaoyan, et al The First Affiliated Hospital, Guangzhou TCM University, Guangzhou (510405
关彤	Chen Jifan, Zhao Huifang, Shen Xiaoyan, et al The First Affiliated Hospital, Guangzhou TCM University, Guangzhou (510405
方永奇	Chen Jifan, Zhao Huifang, Shen Xiaoyan, et al The First Affiliated Hospital, Guangzhou TCM University, Guangzhou (510405
刘晓玲	Chen Jifan, Zhao Huifang, Shen Xiaoyan, et al The First Affiliated Hospital, Guangzhou TCM University, Guangzhou (510405

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中文摘要:

目的：研究通痹灵（ＴＢＬ）对佐剂性关节炎（ＡＡ）大鼠滑膜成纤维细胞增殖及滑膜细胞分泌白细胞介素１（ＩＬ１）、肿瘤坏死因子α（ＴＮＦ α）和前列腺素Ｅ２（ＰＧＥ２）的影响。方法：采用组织块培养的方法获得滑膜成纤维细胞，检测原代培养大鼠滑膜细胞培养上清对滑膜成纤维细胞增殖反应的影响，以及滑膜细胞培养上清中ＩＬ１、ＴＮＦ α的活性和ＰＧＥ２的含量。结果：ＴＢＬ可以明显抑制ＡＡ大鼠滑膜成纤维细胞增殖（Ｐ＜０００１），下调ＡＡ大鼠滑膜细胞分泌ＩＬ１、ＴＮＦ α和ＰＧＥ２（Ｐ＜００１）；消炎痛对滑膜成纤维细胞的增殖具有明显的刺激作用（Ｐ＜０００１），虽能显著抑制ＡＡ大鼠滑膜细胞分泌ＰＧＥ２，但进一步上调了ＩＬ１、ＴＮＦ α的产生（Ｐ＜００１）。结论：ＴＢＬ治疗ＲＡ的机理之一可能是通过下调滑膜细胞的分泌功能，使ＡＡ大鼠滑膜成纤维细胞的过度增殖恢复正常

英文摘要:

Objective: To study the effect of Tongbiling (TBL) on the proliferation of synovial fibroblast and interleukin 1 (IL 1), tumor necrosis factor α (TNF α) and postaglandin E 2 (PGE 2) secretion by synoviocytes in adjuvant arthritis (AA) rats. Methods: Synovial fibroblasts was derived from culture of tissue piece. The effect of primary synoviocyte culture supernatants on the fibroblast proliferation were assayed and IL 1, TNF α bioactivity and PGE 2 content of supernatants of cultured synoviocytes were measured. Results: TBL could inhibit significantly the synovial fibroblast proliferation (P<0 001), and down regulated IL 1, TNF α and PGE 2 productions (P<0 01); indomethacin could promote obviously the synovial fibroblast proliferation (P<0 001). It inhibited significantly PGE 2 production, but further up regulated the IL 1 and TNF α secretion by synoviocytes (P<0 01). Conclusion: The therapeutical effect of TBL on AA might be associated with its down regulating the secretory function of synoviocyte, then restoring the abnormal proliferation of fibroblast to normal levels.

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