颅脑损伤后钙、钙调蛋白的变化及三七总皂甙的治疗作用

目的：探讨颅脑损伤后神经细胞钙超载及钙－钙调蛋白复合物形成与三七总皂甙的治疗作用。方法：应用放射免疫法、原子吸收分光光度法测定动物血及脑组织中钙（Ｃａ２＋）、钙调蛋白（ＣａＭ）含量，观察三七总皂甙对动物脑损伤后上述指标改变的影响。结果：血中Ｃａ２＋、ＣａＭ与脑中Ｃａ２＋、ＣａＭ呈显著正相关，三七总皂甙显著降低血与脑组织中Ｃａ２＋、ＣａＭ的含量。结论：三七总皂甙能阻滞脑损伤后神经细胞内钙超载，阻断Ｃａ２＋－ＣａＭ复合物形成，对颅脑损伤具有一定的保护作用，血中ＣａＭ是评价神经细胞内Ｃａ２＋超载的客观指标

英文摘要:

Objective: To explore the change of nerve cell Ca 2+ overload, Ca 2+ CaM and the effect of Panax Notoginseng saponin (PNS) on it in craniocerebral injury. Methods: Blood and brain contents of Ca 2+ , CaM were examined by radio immunoassay (RIA) and atomic absorption spectrometry(AAS), and effect of PNS on these parameters was observed in craniocerebral injury rabbits. Results: The levels of Ca 2+ and CaM in blood and those in brain were significantly positively correlated. PNS could reduce Ca 2+ and CaM contents in blood and in cerebral tissue. Conclusion: PNS could block the Ca 2+ overload and Ca 2+ CaM complex production in nerve cell after cranial cerebral injury, thereby to protect the injured brain in a certain degree. Blood CaM level is an objective index for assessment of Ca 2+ overload in nerve cell.

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