复方丹参滴丸对冠心病患者循环内皮细胞的内皮素基因表达的影响

作者	单位
冯培芳	Feng Peifang, Qin Nanping, Qin Yuguo, et al Affiliated Hospital of Zhejiang College of TCM, Hangzhou (310006
秦南屏	Feng Peifang, Qin Nanping, Qin Yuguo, et al Affiliated Hospital of Zhejiang College of TCM, Hangzhou (310006
覃宇果	Feng Peifang, Qin Nanping, Qin Yuguo, et al Affiliated Hospital of Zhejiang College of TCM, Hangzhou (310006
程志刚	Feng Peifang, Qin Nanping, Qin Yuguo, et al Affiliated Hospital of Zhejiang College of TCM, Hangzhou (310006
冯久贤	Feng Peifang, Qin Nanping, Qin Yuguo, et al Affiliated Hospital of Zhejiang College of TCM, Hangzhou (310006

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中文摘要:

目的：探讨复方丹参滴丸（ＤＳＰ）对外周血循环内皮细胞ＥＴ－１基因表达的影响。方法：将７０例稳定型心绞痛患者随机分为治疗组（用复方丹参滴丸治疗）及对照组（用消心痛治疗），采用逆转录—聚合酶链反应（ＲＴＰＣＲ）方法于治疗前后测定外周血循环内皮细胞ＥＴ１基因表达，并与３０名健康人作治疗前比较。结果：７０例心绞痛患者６９例检出５４６ｂｐ阳性条带，健康者中无１例阳性。经ＤＳＰ治疗后的２９例中有６例未检到阳性条带，而对照组中无１例转阴。经ＤＳＰ治疗后，ＥＴ１ＰＣＲ产物的相对量（吸收光密度）明显减少，与对照组比较，有显著性差异（Ｐ＜００５）。结论：ＤＳＰ能直接抑制外周血内皮细胞ＥＴ１基因表达。

英文摘要:

Objective: To explore the effect of composite salviae dropping pill (CSDP) on endothelin (ET 1) gene expression in circulating endothelial cells. Methods: Seventy cases of stable angina pectoris were randomly divided into 2 groups, the CSDP group and the isosorbide dinitrate (ID) group. They were treated with CSDP and ID respectively, their ET 1 gene expression in endothelial cells of peripheral circulation was measured before and after treatment by reverse transcriptase Polymerase Chain Reaction and compared between the two groups as well as with that of 30 healthy subjects. Results: Electrophoresis banding of 546 bp cDNA procured from 69 cases of 70 patients was positive, while no positive banding was obtained from healthy subjects. Six cases from the 29 patients treated with CSDP had their banding turned to negative, while in the ID group, no one had turned to negative after treatment. And ET 1 PCR product (absorbed optic density) in the CSDP group was markedly lower than that in the ID group, P<0 05. Conclusion: DSP could inhibit ET 1 gene expression in endothelial cells of peripheral circulation directly.

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