中药天癸方对雄激素致不孕大鼠血leptin及垂体促性腺激素的影响

目的：探讨中药天癸方对雄激素致不孕大鼠（ＡＳＲ）肥胖及生殖功能的影响。方法：运用放射免疫法测定天癸方治疗前后血ｌｅｐｔｉｎ、促卵泡激素（ＦＳＨ）、促黄体生成素（ＬＨ）的变化；同时对ＡＳＲ能量状态指标（每日进食量、腹膜后脂肪垫、体重）进行评估。结果：ＡＳＲ呈高代谢、能量失衡肥胖状态，其血ｌｅｐｔｉｎ水平明显增加（Ｐ＜００１），ＦＳＨ、ＬＨ明显低下（Ｐ＜００１）。ｌｅｐｔｉｎ与体重呈正相关（ｒ＝０８９７７，Ｐ＜０００１），与ＦＳＨ、ＬＨ呈负相关（ｒ＝－０７５１７，Ｐ＜０００１；ｒ＝－０８４４４，Ｐ＜０００１）。中药治疗后各内分泌指标均恢复正常。结论：ＡＳＲ有肥胖及低促性腺激素性性腺功能低下无排卵的特点。天癸方同时有减肥及促排卵作用。

英文摘要:

Objective: To explore the effect of Tiangui Recipe (TGR) on obesity and reproductive function in androgen sterilized rats (ASR). Methods: ASR model was established by subcutaneous injection of testosterone propionate to SD female rats of 9 days age. Change of serum leptin, follicle stimulating hormone (FSH) and luteinizing hormone (LH) in model rats of 115 days old before and after feeding TGR was measured by radioimmunoassay (RIA). Meanwhile, criteria of energy state, including diurnal food intake, retroperitoneal fat depot weight and body weight, were measured and evaluated. Results: ASR had the characteristics of high metabolic rate, energy imbalance and obesity. Serum level of leptin increased and FSH, LH decreased significantly (P<0 01) in model rats as compared with the normal rats. Significant positive correlation between leptin level and body weight (r=0 8977,P<0 001) was observed, and negative correlation was seen between FSH, LH and leptin (r=-0 7517,P<0 001;r=-0 8444,P<0 001, respectively). The endocrinous changes of ASR all returned to normal range after the TGR treatment. Conclusion: Obesity and concerned hypogonadotropic hypogonadal anovulation were expressed in ASR. TGR may play an important role in reducing weight and inducing ovulation.

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