补肾与活血复方调节老年鼠T细胞凋亡的对比研究

目的 :研究老年肾虚T细胞功能减退的机理以及补肾延缓衰老的免疫学基础。方法 :采用电镜、DNA凝胶电泳及TUNEL标记的流式细胞检测技术 ,对各组大鼠抗CD3单抗激活诱导的T细胞凋亡进行定性、定量分析。结果 :老年组激活诱导的T细胞凋亡百分率为 47 0 % ,年轻组为 2 2 2 % (P <0 0 1) ;补肾组为 31 2 % ,明显低于老年组 (P <0 0 5 ) ;活血组T细胞凋亡百分率为 43 3% ,与老年组比较无显著性差异。结论 :激活诱导的T细胞过多凋亡是老年肾虚T细胞功能减退的重要机理 ,下调激活诱导的T细胞凋亡可能是补肾延缓免疫衰老的主要途径。

英文摘要:

Objective: To investigate the mechanism responsible for the observed reduction in T cell function of the Kidney Deficiency Syndrome. Methods: After 18th activation with anti CD 3 mAb, the qualitative and quantitative analysis of apoptoses in T cells were performed by using DNA gel electrophoresis, TdT mediated dUTP nick end labelling (TUNEL) and flow cytometry. Results: The percentage of apoptotic cells was 47.0% in the old and 22.2% in the young group (P<0 01). In the Kidney tonic group, the mean percentage of apoptotic cells was 31.2%, significantly lower than that in the aged group (P<0 05). The mean percentage of apoptotic cells in the promoting blood circulation group was 43.3% and no significant difference was seen in the percentages of apoptotoic T cells from the promoting blood circulation group compared with the old group. Conclusion: Activation induced excessive apoptosis of T cells is one of the mechanisms elucidating decreased T cell function of the Kidney Deficiency Syndrome and aging, the retardation of immune senescence by Kidney tonic recipe could be accounted for by down regulation of activation induced apoptosis in T cells.

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