贺娟,梁怡,王洪图.草果知母汤对点燃癫痫模型鼠大脑NMDA受体基因表达的影响[J].中国中西医结合杂志,1999,(10):617-619 |
草果知母汤对点燃癫痫模型鼠大脑NMDA受体基因表达的影响 |
Effect of Caoguo Zhimu Decoction on N-Methyl-D Aspartate Receptor Gene Expression of Epileptic Kindling Model in Rats |
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DOI: |
中文关键词: 草果知母汤 癫痫模型 NMDA受体 基因表达 |
英文关键词:Caoguo Zhimu decoction epileptic model N Methyl D Aspartate receptor gene expression |
基金项目:国家中医药管理局科研基金!No .94A1 1 1 1 |
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中文摘要: |
目的 :探讨草果知母汤抗癫痫机理是否包含着脑组织NMDAR1mRNA表达的变化。方法 :采用戊四唑 (PTZ)点燃癫痫模型 ,利用分子生物学原位杂交法定量分析NMDAR1mRNA的表达。结果 :草果知母汤治疗 4周后 ,点燃模型鼠海马组织CA1、CA2、CA3区NMDAR1mRNA含量明显降低 ,与模型对照组比较有显著性差异 ;而西药抗癫灵组无明显变化。结论 :草果知母汤能降低NMDAR1mRNA的表达 ,从而可能减少NMDA受体的含量 ,而起到抗癫痫的作用。 |
英文摘要: |
Objective: To explore the effect of Caoguo Zhimu decoction (CGZMD) on N methyl D aspartate (NMDA) receptor gene expression of epileptic model on rat brain. Methods: Adopting the pentylenetetrazol (PTZ) kindling model to observe the NMDAR1 mRNA gene expression by situs hybridization. Results: After 28 days of CGZMD treatment, the NMDAR1 mRNA gene expression on CA1, CA2, CA3 part of rat hippocampus reduced obviously, the comparison between experimental group and the model control group showed significant difference (P<0 05). Conclusion: CGZMD decreased NMDAR1 mRNA gene expression, it may be caused the NMDA receptor decline, hence revealed its anti epileptic effect. |
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