黄芪总皂甙对病毒性心肌炎小鼠心肌损伤及肌浆网钙泵的影响

作者	单位
陆曙	南京医科大学第一附属医院心血管病研究所!南京210029
张寄南	南京医科大学第一附属医院心血管病研究所!南京210029
杨笛	南京医科大学第一附属医院心血管病研究所!南京210029
徐晋丹	南京医科大学第一附属医院心血管病研究所!南京210029
陈相健	南京医科大学第一附属医院心血管病研究所!南京210029
方五旺	南京医科大学第一附属医院心血管病研究所!南京210029
马文珠	南京医科大学第一附属医院心血管病研究所!南京210029

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中文摘要:

目的:观察黄芪总皂甙(AS)及黄芪注射液(AI)对病毒性心肌炎(VMC)小鼠心肌损伤的保护作用及其对心肌肌浆网钙泵的影响。方法:腹腔接种柯萨奇B3亲心肌病毒株建立小鼠VMC模型,分成模型组、AS组、AI组及正常对照组,分别观察各组小鼠死亡率、心肌病理变化、血清心肌肌钙蛋白I(cTnI)含量及心肌肌浆网钙泵(SERCA)活力等指标。结果:模型组死亡率高于对照组(P=00042)、AS组及AI组(P<005);cTnI含量显著高于对照组(P<0001),也高于AS组(P<0025)及AI组(P<005)。AS及AI组的心肌坏死和炎症等病理改变则轻于模型组(P<001)。模型组心肌SERCA活力显著低于对照组(P<0001),AS组(P<001)及AI组(P<005)。结论:AS和AI对VMC小鼠心肌损害具有不同程度的保护作用,AS是黄芪治疗VMC的有效成分;改善VMC小鼠心肌SERCA的活力,可能是黄芪及AS保护感染病毒小鼠心肌免受损害的作用机制之一。

英文摘要:

To investigate the effects on myocardial injury and sarcoplasmic reticulum (SR) Ca 2+ ATPase of viral myocarditis mice treated with Astragaloside (AS) and Astragalus Injection (AI). Methods: Viral myocarditis model was created by intraperitoneal inoculation with coxsackievirus B 3m (CVB 3m ) solution and were divided into model, AS, AI and normal control groups. The mortality, myocardial pathological changes, serum cardiac troponin I (cTnI) and the activity of myocardial Sarco/Endoplasmic Ca 2+ ATPase (SERCA) were observed. Results: The mortality of model was higher than that of the normal control (P=0.0042), AS and AI (P<0 05). The serum level of cTnI of model was significantly higher than that of the normal control (P<0 001), AS (P<0 025) and AI (P<0 05). The myocardial necrosis and inflammatory changes of AS and AI groups were alleviated than that of model (P<0 01). The activity of myocardial SERCA of model were significantly lower than that of normal control (P<0 001), AS (P<0 01) and AI (P<0 05). Conclusions: AS and AI have some protecting effects on myocardial injury of viral myocarditis mice. AS is the effective component of Astragalus membranaceus in treating viral myocarditis. One of the mechanisms of Astragalus membranaceus and AS for viral myocarditis mice depriving of the myocardial injury may be due to improve the activity of myocardial SERCA in the mice.

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