滋补肝肾、益气活血方药对血管平滑肌细胞增殖及相关基因表达的影响

Effect of Replenishing Liver and Kidney, Supplementing Qi and Activating Blood Circulation Recipe on Proliferation and Relevant Gene Expression of Vascular Smooth Muscle Cells

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DOI：

中文关键词: 滋补肝肾益气活血血清药理学血管平滑肌细胞增殖基因表达

英文关键词:replenishing Liver and Kidney supplementing Qi and activating the blood circulation seropharmacology vascular smooth muscle cell proliferation gene expression

基金项目:国家自然科学基金!No .39470 2 70

作者	单位
韩梅	河北医科大学基础所生化室!石家庄050017
温进坤	河北医科大学基础所生化室!石家庄050017

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中文摘要:

目的:采用血清药理学方法研究滋补肝肾、益气活血方药对内皮素(ET)诱导的自发性高血压大鼠(SHR)和WKY大鼠血管平滑肌细胞(VSMC)增殖及相关基因表达的影响。方法:采用3HTdR掺入实验检测VSMC增殖状态,应用Northern杂交、反转录聚合酶链反应(RTPCR)检测中药血清对VSMC诱导型一氧化氮合酶(iNOS)基因和ET诱导的cjun、增殖细胞核抗原(PCNA)基因表达活性的影响。结果:中药血清可明显抑制ET对VSMC的促丝裂作用(P<005);抑制原癌基因cjun和PCNA基因表达,促进iNOS转录。结论:滋补肝肾、益气活血方药通过调节iNOS和细胞增殖相关基因的表达活性进而阻止VSMC增殖。

英文摘要:

Objective: To study the effect of replenishing Liver and Kidney, supplementing Qi and activating blood circulation recipe (TCM) on the proliferation and relevant gene expression of vascular smooth muscle cells (VSMC). Methods: Using 3?H TdR incorporation to investigate the VSMC proliferation, using Northern blotting and reverse Polymerase Chain Reaction (RT PCR) to detect the effect of serum with TCM on induced NO synthase (iNOS), endothelin (ET) induced c jun and proliferation cell nucleus antigen (PCNA) gene expression. Results: The 3?H TdR incorporation value of VSMC treated by serum with TCM was lower than that of control serum (P<0 05). Rich iNOS mRNA was detected in the VSMC stimulated by serum with TCM. Expressions of c jun and PCNA gene induced by ET in the VSMC were significantly inhibited by serum with TCM. Conclusion: The TCM could significantly inhibit VSMC proliferation.

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