炎症因子及益肾活血泄浊汤对大鼠肾小球系膜细胞生长的影响

作者	单位
楚非	CHU Fei, WEI Min, WANG Qian, et al Beijing Medical University, Beijing (100083
魏民	CHU Fei, WEI Min, WANG Qian, et al Beijing Medical University, Beijing (100083
王谦	CHU Fei, WEI Min, WANG Qian, et al Beijing Medical University, Beijing (100083
张悦	CHU Fei, WEI Min, WANG Qian, et al Beijing Medical University, Beijing (100083
严京	CHU Fei, WEI Min, WANG Qian, et al Beijing Medical University, Beijing (100083

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中文摘要:

目的:探讨部分炎症因子及益肾活血泄浊汤对体外培养大鼠肾小球系膜细胞生长的影响。方法:应用脂多糖(LPS)及白细胞介素6(IL6)作为刺激因子,观察其促系膜细胞增殖的作用;并采用血清药理学方法,提取动物的含药血清作用于上述受刺激的系膜细胞,观察益肾活血泄浊汤对系膜细胞增殖的影响。结果:LPS和IL6具有明显促进系膜细胞增殖的作用,并呈一定的量效和时效关系;而益肾活血泄浊汤可显著拮抗LPS和IL6的促细胞增殖作用。结论:系膜细胞是益肾活血泄浊汤发挥治疗作用的重要靶细胞,这可能是该方防治慢性肾小球肾炎发展的机制之一。

英文摘要:

To explore the part of inflammatory factors (cytokines) and Yishen Huoxue Xiezhuo (YSHXXZ) Decoction on the proliferation of extracorporeal cultured mesangial cells (MCs) in rats. Methods: The effects of lipopolysaccharide (LPS) and interleukin 6 (IL 6) as stimulating factors, its action on the proliferation of rat MCs were investigated by using the technique of 3 H TdR incorporation. Meanwhile, adopting serum pharmacology assay, the medicated serum of rat, containing YSHXXZ decoction was extracted and its effects on the growth of MCs were also studied. Results: LPS and IL 6, in a dose dependent and time dependent manner, could markedly stimulate the MCS proliferation, while this stimulatory effects could be strongly antagonized by the serum containing YSHXXZ decoction. Conclusion: Mesangial cell is the main target cell of the action of YSHXXZ decoction, and the inhibition on MCs might be one of the mechanisms of the YSHXXZ decoction in preventing the progression of chronic glomerulonephritis.

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