大黄素对狼疮性肾炎成纤维细胞生物学行为的影响

作者	单位
刘冠贤	LIU Guanxian, YE Rengao, TAN Zhiming, et al. Department of Nephrology, Huizhou Central People’s Hospital, Guangdong (516001
叶任高	LIU Guanxian, YE Rengao, TAN Zhiming, et al. Department of Nephrology, Huizhou Central People’s Hospital, Guangdong (516001
谭志明	LIU Guanxian, YE Rengao, TAN Zhiming, et al. Department of Nephrology, Huizhou Central People’s Hospital, Guangdong (516001
钟伟强	LIU Guanxian, YE Rengao, TAN Zhiming, et al. Department of Nephrology, Huizhou Central People’s Hospital, Guangdong (516001
杨永铭	LIU Guanxian, YE Rengao, TAN Zhiming, et al. Department of Nephrology, Huizhou Central People’s Hospital, Guangdong (516001
张国强	LIU Guanxian, YE Rengao, TAN Zhiming, et al. Department of Nephrology, Huizhou Central People’s Hospital, Guangdong (516001
方敬爱	LIU Guanxian, YE Rengao, TAN Zhiming, et al. Department of Nephrology, Huizhou Central People’s Hospital, Guangdong (516001

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中文摘要:

目的：观察中药大黄素（ｅｍｏｄｉｎ）对狼疮性肾炎（ＬＮ）肾间质成纤维细胞生物学行为的影响。方法：将ＬＮ患者肾活检组织体外培养分离出成纤维细胞，分别用３Ｈ－ＴｄＲ掺入法和流式细胞术检测不同浓度的大黄素对人肾成纤维细胞增殖、凋亡及ｃ－ｍｙｃ蛋白水平表达情况。结果：大黄素能明显抑制人肾成纤维细胞的分裂增殖，并通过促使ｃ－ｍｙｃ蛋白高水平表达诱导细胞发生凋亡。结论：大黄素有抑制成纤维细胞增殖和促其凋亡的作用，从而达到减轻ＬＮ肾间质纤维化病变。

英文摘要:

To observe the effects of emodin on human fibroblasts in culture of kidney in patients with lupus nephritis (LN). Methods: Fibroblasts were isolated from culture of kidney of LN patients, and the effect of emodin on 3H-TdR incorporated rate of fibroblasts was observed. The apoptosis and c-myc gene expression were detected in the same time by flow cytometry. Results: Emodin could inhibit the proliferation of fibroblasts, and promote the programmed cell death through upregulate c-myc gene expression in human renal fibroblasts. Conclusion: Emodin can inhibit proliferation and promote apoptosis of fibroblasts, which may be important in ameliorate interstitial fibrosis, thus improving prognosis of LN.

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