中药复圣散对高脂血症大鼠脑缺血再灌注后脑内若干神经肽含量的影响

目的：阐明脑缺血后部分神经肽类的变化规律以及中药复圣散对神经肽的调节作用和对脑缺血性损伤的保护机制。方法：通过高脂饮食喂养复制食饵性高脂血症大鼠，在此基础上采用反复脑缺血再灌流手术造成大鼠脑损伤模型，以特异性放射免疫分析法检测脑组织匀浆中各神经肽含量。结果：大鼠脑缺血再灌注后１天，脑内神经肽内皮素（ＥＴ－１）、神经肽Ｙ（ＮＰＹ）、降钙素基因相关肽（ＣＧＲＰ）的含量分别比正常对照组升高，生长抑素（ＳＳ）含量则下降（均Ｐ＜０．０１）；术后７天，ＣＧＹ、ＣＧＲＰ、ＳＳ等均有恢复，但ＥＴ浓度仍持续增高；经复圣散预防和治疗性给药后，各项指标均有不同程度的好转（Ｐ＜０．０５，Ｐ＜０．０１）。结论：提示脑缺血性损伤与脑内ＥＴ、ＮＰＹ、ＣＧＲＰ和ＳＳ等神经肽类释放失衡密切相关，而中药复圣散对脑内神经肽含量有一定的调节作用，推测这可能是该方防治脑血管病的主要作用机制之一。

英文摘要:

To observe the changes of some neuropeptides and the effect of Fusheng powder (FSP) on neuropeptides in rat’s brains in a stable cerebral ischemia and reperfusion (I/L) model. Methods: The models of rat’s brain injured were established by repeated cerebral I/R in rats with hyperlipidemia. Radioimmunoassay (RIA) was performed to determine the level of neuropeptides. Results: After 1 day of I/R, compared with the control group the contents of cndothelin-1(ET-1), calcitonin gene related peptide (CGRP) and neuropep- tide Y (NPY) in the model animals were significantly increased by 24. 3%, 33. 7% and 51. 86% respectively, while the level of somatostatin (SS) decreased by 37. 86 % (all P< 0. 01 ). Meanwhile after FSP treatment, the contents of neuropeptides were alleviated respectively (P< 0.05, P< 0.01 ). Apart from the ET, the releases of CGRP, NPY and SS were all recovered in different degree after 7 days of I/R. Conclusions: There were obvious imbalance of neuropeptides in rat’s brains after cerebral I/R and the FSP might antagonize ischemic injury of brain through modulating neuropeptides, which may be one of the therapeutical mechanism in treating cerebral vascular diseases with FSP.

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