银杏叶制剂治疗肺间质纤维化的实验研究

目的：探讨银杏叶制剂（百路达）对博莱霉素A₅所致大鼠肺间质纤维化的治疗作用及机制。方法：以博莱霉素A₅所致大鼠肺间质纤维化为模型，观察百路达治疗后病理、胶原蛋白、核因子kB活性、转化生长因子-β（TGF-β）mRNA表达及蛋白水平的变化。结果：百路达治疗组肺泡炎、纤维化病变均较博莱霉素模型组减轻（P＜0．05），其胶原蛋白含量较模型组减少（P＜0．01），百路达治疗组1周肺泡巨噬细胞核因子kB（N－kB）活性明显降低（47．3％），TGF-βmRNA表达及其蛋白水平均有所降低（P＜0．05）。结论：百路达治疗肺间质纤维化确有疗效。其机制可能通过抑制核因予kB活性，减少了TGF-βmRNA表达及蛋白产生，使炎症及纤维化病变减轻。

英文摘要:

0bjective: To evaluate the therapeutic effect and mechanism of folium Ginkgo biloba (FGB) in treating pulmonary interstitial fibrosis (PIF) induced by bleomycin in rats. Methods: PIF models of bleomycin-A5-in- duced pulmonary fibrosis were established in rats, they were treated by Bailuda, a preparation of FGB, and the pathological changes, collagen protein level, nuc1ear factor kB(NF-kB) activity, transforming growth factor 9 (TGF-8) mRNA expression and protein level of the lung tissue were measured. Results: In the Bailuda treated group after treatment, amelioration of the pulmonary alveolitis and fibrosis were shown in pathological section (P < 0. O5 ) and collagen protein content was lesser (P < O. 01 ) as compared with those in the model control group. After l week of Bailuda treatment, the NF-kB activity of pulmonary alveola macrophage lowered by 47.3 %, and levels of TGF-8 mRNA expression and protein were all decreased (P < O. O5 ). Conclusions: Bailuda has definite effect in treating PIF. The mechanism may be through inhibiting the activity of NF-kB, decreasing TGF-9 mRNA expression and protein, so as to ameliorate the inflammation and fibrosis.

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