地黄寡糖的吸收动力学研究

目的：研究地黄寡糖在小鼠及大鼠体内的吸收和吸收部位。方法：用高效液相色谱／柱后荧光衍生化法测定小鼠口服和静脉注射水苏糖后的血药浓度；大鼠原位肠灌流模型研究水苏糖的吸收和吸收部位；肠抑菌实验观察水苏糖在肠道内的代谢。结果：口服吸收快但很少，生物利用度为3．82％。在胃、十二指肠、空肠和回肠的吸收率分别为6．03％、13．80％、8.33％和0．53％，在大肠内不吸收。正常组及卡那霉素抑菌组的小鼠灌服水苏糖3h后，两组肠内的水苏糖残存量分别为8．6％和93．4％。结论：水苏糖口服吸收少，主要吸收部位在小肠上部，未吸收的水苏糖在肠内被细菌破坏。

英文摘要:

Objective: To study the intestinal absorption and absorption site of stachyose, one of the oligosaccharides extracted from Rehmannia, in mice and rats. Methods: Plasma concentreation of stachyose was measured by high performance liquid chromatography (HPLC) with postcolumn fluorescence derivatization after administra tion of stachyose to mice by oral or intravenous injection. The absorption of stachyose in different sections of di gestive tract was evaluated using the intestine loop in situ technique. The kanamycin treated mice were used to study the metabolism of stachyose in intestine. Results: Absorption of stachyose in intestine after oral adminis tration was quick but low, the bioavailability being 3. 82%. The absorption rate in stomach, duodenum, je junum and ileum was 6. 03%, 13. 80%, 8. 33% and 0. 53% respectively, no absorption was tested in large in testine. The residual rate of stachyose in the intestine of normal mice and kanamycin treated mice was 8. 6% and 93. 4% respectively. Conclusion: The intestinal absorption rate of stachyose is low, the main absorption site is the upper portion of small intestine. The unabsorbed stachyose is metabolized by the intestinal microflora.

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