韩谷鸣,姚倩,李洪莲,王永平.穿心莲黄酮对血小板活化反应的抑制作用及机理研究[J].中国中西医结合杂志,2000,(7):527-529 |
穿心莲黄酮对血小板活化反应的抑制作用及机理研究 |
nhibitory Effect of Panicoin on Platelet Activation and Its Mechanism |
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DOI: |
中文关键词: 穿心莲黄酮 血小板活化 花生四烯酸 cAMP |
英文关键词:Panicoin platelet activation arachidonic acid cyclic AMP |
基金项目:江苏省科委资助项目!(BJ91065) |
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中文摘要: |
目的:探讨穿心莲黄酮抑制血小板活化反应的途径和可能机理。方法:观察穿心莲黄酮对二磷酸腺苷(AD P)、肾上腺素、花生四烯酸(AA)、凝血酶诱导的血小板聚集、血栓素B2(TXB2)释放、P-选择素(GMP-140)及血小板内环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)水平的影响。结果:(1)穿心莲黄酮可明显抑制ADP、AA、肾上腺素诱导的血小板聚集,并呈明显的正相关;(2)穿心莲黄酮可明显降低凝血酶诱导的GMP-140水平;(3)穿心莲黄酮可明显抑制AA诱导的血小板TXB2的释放,并可明显升高血小板内cAMP的含量,对cGMP含量无明显影响。结论:穿心莲黄酮具有较强的抑制血小板活化反应和抗血栓形成的作用,升高血小板内的cAMP水平可能为其作用机理之一。 |
英文摘要: |
To explore the inhibitory pathway and the possible mechanism of panicoin on platelet activation. Methods: The effect of panicoin on platelet aggregation, thromboxane B2 release, P-selectin (GMP-140) count, levels of cyclic AMP and cyclic GMP induced by ADP, epinephrine, arachidonic acid (AA) or thrombin were observed. Results: (1) Panicoin significantly inhibits platelet aggregation induced by ADP, arachidonic acid and epinephrine in a dose-dependent manner; (2) It reduces the P-selectin number on platelet surface which was stimulated by thrombin; (3) It inhibits TXB2 release induced by AA and increases the platelet cyclic AMP level, while cyclic GMP level was not changed. Conclusion: Panicoin shows a potent inhibition on platelet activation and antithrombotic effect. The mechanisms seem to be related with the elevation of platelet cyclic AMP. |
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