三氧化二砷诱导HL-60细胞凋亡的实验研究

作者	单位
王展翔	WANG Zhanxiang, XU Yonggang, LIAO Junxian, et al (Department of Hematology, Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing (100091)
许勇钢	WANG Zhanxiang, XU Yonggang, LIAO Junxian, et al (Department of Hematology, Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing (100091)
廖军鲜	WANG Zhanxiang, XU Yonggang, LIAO Junxian, et al (Department of Hematology, Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing (100091)
麻柔	WANG Zhanxiang, XU Yonggang, LIAO Junxian, et al (Department of Hematology, Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing (100091)
周霭祥	WANG Zhanxiang, XU Yonggang, LIAO Junxian, et al (Department of Hematology, Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing (100091)

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中文摘要:

目的：探讨三氧化二砷（Ａｓ_２Ｏ_３）治疗白血病的疗效机理。方法：以ＨＬ－６０细胞株为体外模型，终浓度为０．２０μｍｏｌ／ＬＡｓ２_Ｏ_３作用２４、４８ｈ后，用ＰＩ／ＡｎｎｅｘｉｎＶ－ＦＩＴＣ双标记，流式细胞术观察Ａｓ_２Ｏ_３对ＨＬ－６０细胞凋亡及其对细胞株端粒酶活性的影响。结果：Ａｓ２_Ｏ_３作用于ＨＬ－６０细胞后ＰＩ阴性／ＡｎｎｅｘｉｎＶ－ＦＩＴＣ阳性细胞增多，提示细胞发生凋亡，ＤＮＡ周期分析显示主要作用在细胞Ｇ_２－Ｍ期，４８ｈ对端粒酶有轻微抑制作用。结论：Ａｓ_２Ｏ_３主要通过诱导细胞凋亡杀伤白血病细胞。

英文摘要:

To explore the mechanism of arsenic （arsenic trioxide, As₂O₃） in treating leukemia. Methods: The in vitro effect of arsenic trioxide on HL-60 model double-labelled with PI/AnnexinV-FITC was studied with flow cytometry. Function on telomerase activity was also observed. Results: Alter the effect of arsenic on HL60 cell line, PI negative/ AnnexinV- FITC positive cells increased, it mainly acts at t he G2 - M phase that suggests the apoptosis takes place,. mild inhibition on telomerase was also found 48 hours later. Conclusion: Apoptosis induced by arsenic trioxide was the main mechanism in killing or inhibiting leukemic cells.

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