补肾、活血复方对老年大鼠T细胞凋亡相关基因Fas、FasL转录的影响

作者	单位
郑振	ZHENG Zhen, SHEN Ziyin, ZHENG Zhongcheng, et al. Institute of Integrated Traditional Chinese and Western Medicine, Shanghai Medical University, Shanghai (200040
沈自尹	ZHENG Zhen, SHEN Ziyin, ZHENG Zhongcheng, et al. Institute of Integrated Traditional Chinese and Western Medicine, Shanghai Medical University, Shanghai (200040
郑仲承	ZHENG Zhen, SHEN Ziyin, ZHENG Zhongcheng, et al. Institute of Integrated Traditional Chinese and Western Medicine, Shanghai Medical University, Shanghai (200040
严明达	ZHENG Zhen, SHEN Ziyin, ZHENG Zhongcheng, et al. Institute of Integrated Traditional Chinese and Western Medicine, Shanghai Medical University, Shanghai (200040
徐蔚晶	ZHENG Zhen, SHEN Ziyin, ZHENG Zhongcheng, et al. Institute of Integrated Traditional Chinese and Western Medicine, Shanghai Medical University, Shanghai (200040
黄辉	ZHENG Zhen, SHEN Ziyin, ZHENG Zhongcheng, et al. Institute of Integrated Traditional Chinese and Western Medicine, Shanghai Medical University, Shanghai (200040

摘要点击次数: 1324

全文下载次数: 805

中文摘要:

目的 :探讨补肾复方下调老年大鼠T细胞凋亡的分子机理。方法 :采用RNA酶保护技术 ,测定各组Fas、FasL基因的mRNA水平 ,并以GAPDH为内对照基因 ,对各组Fas、FasL基因的转录进行半定量分析。结果 :老年对照组Fas/GAPDH、FasL/GAPDH的积分光密度值分别为 0 81± 0 17、0 84± 0 12 ;年轻对照组分别为 0 36± 0 16、0 2 6± 0 12。两组比较差异有显著性。活血复方组Fas/GAPDH、FasL/GAPDH的积分光密度值分别为 0 99± 0 36、0 94± 0 33,与老年对照组比较 ,差异无显著性 (P >0 0 5) ;两个补肾组Fas/GAPDH为 0 78± 0 11和 0 78± 0 2 0 ,与老年对照组比较差异无显著性 (P >0 0 5) ;而两个补肾组FasL/GAPDH的积分光密度值为 0 71± 0 2 1和 0 68± 0 15,低于老年对照组 (P <0 0 1)。结论 :补肾复方对T细胞FasL基因的转录具有一定的负调控作用 ,这是补肾复方下调老年大鼠T细胞过度凋亡的分子机理之一

英文摘要:

Objective: To probe the molecular mechanism for down regulation of T cell apoptosis by Kidney tonifying compound (KTC) in aged rats. Methods: GAPDH was used as an internal control gene, and semi quantitative analysis of the transcription of Fas and FasL genes was performed by using RNase protection assay. Results: The Fas/GAPDH and FasL/GAPDH optical density (OD) ratios was 0.81±0.17 and 0.84±0.12 in the aged control group, and 0.36±0.16 and 0.26±0.12 in the young control group. the difference between the two groups was significant. In the circulation promoting group, the parameters were 0.99± 0.36 and 0.94±0.33 which were not significantly different as compared with those in the aged control group (P>0 05). In the two Kidney tonifying groups, the Fas/GAPDH OD ratio was 0.78±0.11 and 0.78±0.20, respectively, and no significant difference was observed as compard with those in the aged control group. However the FasL/GAPDH OD ratios in the two kidney tonifying group were 0.71±0.21 and 0.68±0.15 , which were lower than those in the aged control group (P<0 01). Conclusion: The negative regulation of FasL gene expression may be one of the molecular mechanisms of KTC in down regulating excessive apoptosis of T cell in aged rats.

关闭