| 陈德昌,乔林,景炳文,杨兴易,李红江.大黄对烫伤大鼠肠粘膜上皮细胞线粒体单价泄漏的影响[J].中国中西医结合杂志,2000,(11):849-852 |
| 大黄对烫伤大鼠肠粘膜上皮细胞线粒体单价泄漏的影响 |
| Effect of Rhubarb on Oxygen Radicals Leakage from Mitochondria of Intestinal Mucosa in Burned Rats |
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| DOI： |
| 中文关键词: 烧伤 大鼠 线粒体 小肠 氧自由基 |
| 英文关键词:burn rat mitochondria small intestine oxygen free radicalt |
| 基金项目:国家自然科学基金资助项目!(No .39370 666) |
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| 摘要点击次数: 1223 |
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| 中文摘要: |
| 目的 :研究大黄对烫伤大鼠肠粘膜上皮细胞线粒体单价泄漏的影响。方法 :采用大鼠背部烫伤模型 ,监测其细胞色素氧化酶的活力 ,同时测定血浆、肝脏、小肠粘膜及其线粒体内过氧化物歧化酶 (SOD)和丙二醛 (MDA)水平。结果 :烫伤后大鼠小肠粘膜上皮细胞线粒体内细胞色素氧化酶的活力随时间而降低 ,大黄能显著提高细胞色素氧化酶的活力。烫伤后血浆、肝脏、小肠粘膜及其线粒体内SOD大量消耗 ,MDA明显增加 ,而大黄能显著降低组织内SOD的消耗和MDA产生。结论 :大黄能提高烫伤大鼠小肠粘膜上皮细胞线粒体内SOD和细胞色素氧化酶的活力 ,降低线粒体内单价泄漏 |
| 英文摘要: |
| Objective: To investigate the effects of rhubarb on oxygen radicals leakage from the mitochondria of intestinal mucosa in burned rats. Methods: The activity of cytochrome oxidase and superoxide dismutase (SOD) in mitochondria isolated from intestinal mucosa of burn model of rats was used to evaluate the oxygen radicals leakage from the mitochondria. And the SOD and malondialdehyde (MDA) levels in plasma, liver, intestinal mucosa and its mitochondria were also determined. Results: After burn injury, the activity of cytochrome oxidase in intestinal mucosa decreased as the time elapses, rhubarb could improve it obviously. Furthermore, rhubarb could reduce the massive consumption of SOD and production of MDA obviously increased in plasma, liver, intestinal mucosa and its mitochondria after burning. Conclusion: Rhubarb could enhance the activity of cytochrome oxidase and SOD in small intestine mucosal epithelia in burned rats, thus the oxygen radicals leakage from the mitochondria to be reduced. |
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