刘春华,钱冠清,刘会齐,刘晓辉.银杏叶提取物抑制轻度修饰低密度脂蛋白诱导的血管内皮细胞与单核细胞粘附的实验研究[J].中国中西医结合杂志,2000,(12):917-919 |
银杏叶提取物抑制轻度修饰低密度脂蛋白诱导的血管内皮细胞与单核细胞粘附的实验研究 |
Experimental Study on Suppressive Effect of Ginkgo Extract on Adhesion of Vascular Endothelial Cell to Monocyte Induced by Minimally Modified Low Density Lipoprotein |
免费下载全文 查看/发表评论 下载PDF阅读器 |
|
DOI: |
中文关键词: 内皮细胞 轻度修饰低密度脂蛋白 细胞粘附分子 银杏叶提取物 |
英文关键词:minimally modified low density lipoprotein endothelial cell adhesion molecule Ginkgo extract |
基金项目:国家自然科学基金!重点项目资助 (No .39730 2 2 0 ) |
|
摘要点击次数: 1331 |
全文下载次数: 1311 |
中文摘要: |
目的 :观察银杏叶提取物 (EGb)对轻度修饰低密度脂蛋白 (MM LDL)诱导的人脐静脉内皮细胞 (HUVEC)与人类单核细胞系U937粘附功能的影响。方法 :利用计数法观察HUVEC与U937细胞的粘附率 ;用ELISA方法检测MM LDL作用后HUVEC膜表面粘附分子血管细胞粘附分子 1(VCAM 1)、细胞间粘附分子 1(ICAM 1)及P选择素 (P selectin)的表达。结果 :MM LDL( 75μg/ml)作用HUVEC 4h后 ,其对U937细胞粘附明显增加 (P <0 0 1) ,HUVEC膜表面未见VCAM 1、ICAM 1及P selectin表达上调 ,作为阳性对照重组肿瘤坏死因子α(rTNFα) 5 0ng/ml可显著诱导以上 3种粘附分子表达。中药EGb剂量依赖性地抑制MM LDL诱导HUVEC与U937细胞粘附。结论 :EGb能保护HUVEC ,减少MM LDL对其活化 ,可能有利于延缓动脉粥样硬化 (AS)早期进展。MM LDL诱导的HUVEC与U937粘附不是通过ICAM 1,VCAM 1及P selectin介导的。 |
英文摘要: |
To observe the effect of Ginkgo extract on the interaction between minimally modified low density lipoprotein (MM-LDL) induced human umbilical vein endothelial cell (HUVEC) and U937 monocyte-like cell line. Methods: The adhesive percentage between HUVEC and U937 by counting and the expression of VCAM-1, ICAM-1, P-selectin by ELISA. Results: Treatment of HUVEC with MM-LDL (75μg/ml) for 4 hrs could significantly increase the adhesion of U937 to HUVEC (P<0.01) and Ginkgo extract could suppress the adhesion in dose-dependent manner. The surface expression of VCAM-1, ICAM-1, P-selectin was not induced by MM-LDL, while recombination tumor necrosis factor-α 5.0 ng/ml, as a positive control, could do. Conclusion: Ginkgo extract afforded protection against HUVEC damage induced by MM-LDL and the adhesion of monocytes to endothelial cells induced by MM-LDL is not mediated by VCAM-1, ICAM-1 and P-selectin. |
关闭 |