肾康注射液对肾小管上皮细胞转化生长因子-βmRNA基因表达的影响

作者	单位
赵宗江	ZHAO Zongjiang, LI Hong, ZHANG Xinxue, et al. Beijing University of Traditional Chinese Medicine, Beijing (100029
李宏	ZHAO Zongjiang, LI Hong, ZHANG Xinxue, et al. Beijing University of Traditional Chinese Medicine, Beijing (100029
张新雪	ZHAO Zongjiang, LI Hong, ZHANG Xinxue, et al. Beijing University of Traditional Chinese Medicine, Beijing (100029
傅博	ZHAO Zongjiang, LI Hong, ZHANG Xinxue, et al. Beijing University of Traditional Chinese Medicine, Beijing (100029
叶一舟	ZHAO Zongjiang, LI Hong, ZHANG Xinxue, et al. Beijing University of Traditional Chinese Medicine, Beijing (100029
陈香美	ZHAO Zongjiang, LI Hong, ZHANG Xinxue, et al. Beijing University of Traditional Chinese Medicine, Beijing (100029
叶传蕙	ZHAO Zongjiang, LI Hong, ZHANG Xinxue, et al. Beijing University of Traditional Chinese Medicine, Beijing (100029
牛建昭	ZHAO Zongjiang, LI Hong, ZHANG Xinxue, et al. Beijing University of Traditional Chinese Medicine, Beijing (100029

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中文摘要:

目的 :探讨肾康注射液对体外培养的肾小管上皮细胞LLC PK1转化生长因子 βmRNA(TGF βmRNA)基因表达的影响。方法 :用逆转录聚合酶链反应 (RT PCR)、Northern印迹杂交 (Northernblot)方法 ,以单味大黄注射液为实验对照组 ,检测肾康注射液对肾小管上皮细胞TGF βmRNA基因表达的影响。结果 :肾康注射液可以明显下调肾小管上皮细胞TGF βmRNA基因的表达 ,并呈剂量依赖关系 ,肾康注射液作用明显优于同等含量的单味大黄注射液。结论 :肾康注射液下调肾小管上皮细胞TGF βmRNA基因表达 ,是其防治慢性肾功能衰竭 (CRF)的机理之一。

英文摘要:

To study the effect of Shenkang injection (SKI) on the transforming growth factor (TGF) -βmRNA of LLC-PK 1 renal tubular epithelial cells. Methods: Using reverse transcriptase-polymerase chain reaction (RT-PCR) and Northern Blot to observe the effect of SKI on the TGF-βmRNA of LLC-PK 1 renal tubular epithelial cells, with control group treated with the injection of Rheum officinale extraction. Results: SKI showed the inhibition of TGF-βmRNA expression of renal tubular epithelial cells, which is dose-dependent and was more obvious than that of the Rheum extractive injection with equal dose of drug. Conclusion: The mechanism of delaying chronic renal failure with SKI is achieved by down regulating TGF-βmRNA expression of renal tubular cells

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