张立煌,吴嘉庚,姚航平,李敏伟.抗纤方抑制TGF-β1诱导肝细胞凋亡的实验研究[J].中国中西医结合杂志,2001,(1):37-39 |
抗纤方抑制TGF-β1诱导肝细胞凋亡的实验研究 |
Experimental Study of Inhibitory Effect of Kangxian Recipe on TGF-β1 Induced Hepatocyte Apoptosis |
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DOI: |
中文关键词: 抗纤方 肝细胞 凋亡 TGFβ1 |
英文关键词:Kangxian Recipe hepatocyte apoptosis TGF-β 1 |
基金项目:国家自然科学基金重点项目资助课题!(No .39730 4 2 0 ) |
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中文摘要: |
目的 :探讨抗纤方对TGF β1诱导肝细胞凋亡的抑制作用。方法 :采用细胞生物学等方法 ,利用TGF β1可诱导肝细胞凋亡的特性 ,建立体外实验模型 ,观察抗纤方对TGF β1诱导肝细胞凋亡抑制作用。结果 :实验表明TGF β1以剂量依赖关系诱导肝细胞凋亡 ,当TGF β1浓度在 50 0 pg/ml时可诱导2 2 15细胞凋亡达 63% ,而HepG2细胞凋亡只有 4 2 % ;此时 ,用抗纤方 2 0mg/ml可使 2 2 15细胞凋亡率降至 33% ,HepG2细胞凋亡率降至 2 4 % ,抑制率均在 50 %左右。结论 :抗纤方体外对TGF β1诱导的肝细胞凋亡有明显的抑制作用 |
英文摘要: |
To study the inhibitory effect of Kangxian Recipe (KXR) on TGF-β 1 induced hepatocyte apoptosis. Methods: The in vitro model of hepatocyte apoptosis was established by cell biologic methods, utilizing the characteristics of TGF-β 1 to observe the inhibitory effect of KXR on hepatocyte apoptosis. Results: TGF-β 1 induced apoptosis of hepatocyte in a dose-dependent manner. The apoptosis rate of 2.2.15 cells was 63% when 500 ng/L TGF-β 1 was used, while for HepG2 cell, it was merely 44%. After treatment of 20 ng/L KXR, the apoptosis rate of the two kinds of cell lines lowered to 33% and 24% respectively. The inhibiton rate of both groups was about 50%. Conclusion: KXR had strong inhibitory effect on hepatocyte apoptosis induced by TGF-β 1. |
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