补肾活血中药对雌性幼龄大鼠乳腺发育作用及其机理的实验研究

Effect and Mechanism Study of Kidney Tonifying and Blood Circulation Activating Chinese Drugs on Development of Mammary Glands in Female Juvenile Rats

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DOI：

中文关键词: 乳腺发育补肾活血雌二醇孕酮雌二醇受体孕酮受体

英文关键词:development of mammary gland Kidney tonifying blood circulation activating estradiol progesterone estradiol receptor progesterone receptor

基金项目:

作者	单位
李培英	广州中医药大学!广州510407
欧阳惠卿	广州中医药大学第一附属医院

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中文摘要:

目的 :探讨补肾活血中药对乳腺发育的作用及其机理。方法 :以雌性幼龄大鼠为实验对象 ,以血清激素浓度、乳腺组织雌激素受体和孕激素受体数量及亲和力、乳腺细胞DNA含量及乳腺重量指数等为观察指标 ,探讨研究补肾活血中药 (熟地、枸杞子、仙茅、肉苁蓉、丹参等 )在受体前、受体及受体后各个环节对乳腺发育的影响。结果 :在受体前环节 ,补肾活血中药能够促进血中雌、孕激素水平的同步升高以及生长激素分泌的增加 ;在受体环节 ,补肾活血中药通过对受体直接及间接作用 ,使大鼠乳腺细胞雌、孕激素受体数量增加 ,与激素的亲和力增强 ;在受体后环节 ,补肾活血中药的作用表现为引起乳腺组织DNA合成的增强和乳腺重量指数升高。结论 :补肾活血中药具有促进大鼠乳腺生长发育的作用。

英文摘要:

To study the effect and mechanism of Kidney tonifying and blood circulation activating Chinese drugs (KB) on development of mammary gland. Methods: Using the female juvenile rats as the experimental targets, the level of serum hormone, the amount and affinity of estradiol receptor (ER) and progesterone receptor (PR) as well as the DNA content and relative weight of the rats′ mammary glands were measured to study the effect of KB at the pre-receptor, receptor and post-receptor level. Results: KB markedly enhanced the serum level of estradiol, progesterone and the growth hormone (GH) at pre-receptor level, increased the number and affinity of estradiol and progesterone receptors both in the cytoplasm and nucleus at the receptor level. While at the level of post-receptor, it increased the DNA content and the weight index of mammary glands. Conclusion: The Kidney tonifying and blood circulation activating Chinese drugs are effective in promoting the growth and development of mammary glands.

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